The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 3 - 6 µg/ml.
Use at an assay dependent dilution. PubMed: 19729835
Tissue specificityBrain, testis, ovary, prostate, liver, colon, kidney, and small intestine.
PathwayLipid metabolism; bile acid biosynthesis.
Involvement in diseaseDefects in CYP7B1 are the cause of spastic paraplegia autosomal recessive type 5A (SPG5A) [MIM:270800]. Spastic paraplegia is a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body. Defects in CYP7B1 are the cause of congenital bile acid synthesis defect type 3 (CBAS3) [MIM:613812]. Clinical features include severe cholestasis, cirrhosis and liver synthetic failure. Hepatic microsomal oxysterol 7-alpha-hydroxylase activity is undetectable.
Sequence similaritiesBelongs to the cytochrome P450 family.
ab19043 at 4µg/ml, staining CYP7B1 in Human Breast tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Image shows textured cytoplasm staining in ductal epithelial cells. Steamed antigen retrieval with pH 6 citrate buffer was performed, and HRP-staining was used.
References for Anti-CYP7B1 antibody (ab19043)
This product has been referenced in:
Leuenberger N et al. Sumoylated PPARalpha mediates sex-specific gene repression and protects the liver from estrogen-induced toxicity in mice. J Clin Invest : (2009).
Read more (PubMed: 19729835) »