• Product nameAnti-CYP7B1 antibody
    See all CYP7B1 primary antibodies
  • Description
    Mouse monoclonal to CYP7B1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: CDNNKFISEL RDDFLKFDDK FAYLVSNIPI ELLGNVKSIR EKIIKCFSSE KLAKMQGWSE VFQSRQDVLE KYYVHEDLEI GAHH, corresponding to amino acids 203-286 of human CYP7B1 (NP_004811) with a 26 kDa tag.

  • Positive control
    • CYP7B1 transfected 293T cell lysate. Recombinant tagged human CYP7B1 fragment (the immunogen).



Our Abpromise guarantee covers the use of ab77157 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 58 kDa.
ELISA Use at an assay dependent dilution. Detection limit for recombinant tagged CYP7B1 is approximately 0.3 ng/ml when used as a capture antibody.


  • Tissue specificityBrain, testis, ovary, prostate, liver, colon, kidney, and small intestine.
  • PathwayLipid metabolism; bile acid biosynthesis.
  • Involvement in diseaseDefects in CYP7B1 are the cause of spastic paraplegia autosomal recessive type 5A (SPG5A) [MIM:270800]. Spastic paraplegia is a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body.
    Defects in CYP7B1 are the cause of congenital bile acid synthesis defect type 3 (CBAS3) [MIM:613812]. Clinical features include severe cholestasis, cirrhosis and liver synthetic failure. Hepatic microsomal oxysterol 7-alpha-hydroxylase activity is undetectable.
  • Sequence similaritiesBelongs to the cytochrome P450 family.
  • Cellular localizationEndoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 25 hydroxycholesterol 7 alpha hydroxylase antibody
    • 25-hydroxycholesterol 7-alpha-hydroxylase antibody
    • CP7B antibody
    • CP7B1_HUMAN antibody
    • Cyp7b1 antibody
    • Cytochrome P450 7B1 antibody
    • Cytochrome P450 family 7 subfamily B polypeptide 1 antibody
    • Cytochrome P450 subfamily VIIB polypeptide 1 antibody
    • Oxysterol 7-alpha-hydroxylase antibody
    • Oxysterol 7alpha hydroxylase antibody
    see all

Anti-CYP7B1 antibody images

  • Anti-CYP7B1 antibody (ab77157) at 5 µg/ml + Recombinant tagged human CYP7B1 fragment at 0.2 µg

    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 58 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-CYP7B1 antibody (ab77157) at 5 µg/ml

    Lane 1 : CYP7B1 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 58 kDa
    Observed band size : 58 kDa

References for Anti-CYP7B1 antibody (ab77157)

This product has been referenced in:
  • Zhao YD  et al. De novo synthesize of bile acids in pulmonary arterial hypertension lung. Metabolomics 10:1169-1175 (2014). WB ; Human . Read more (PubMed: 25374487) »

See 1 Publication for this product

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