Overview

  • Product name
  • Description
    Rabbit polyclonal to CysLT2
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region from the 1st extracellular domain of Human CysLT2.

  • Positive control
    • Human Heart tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab115453 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Receptor for cysteinyl leukotrienes. The response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. Stimulation by BAY u9773, a partial agonist, induces specific contractions of pulmonary veins and might also have an indirect role in the relaxation of the pulmonary vascular endothelium. The rank order of affinities for the leukotrienes is LTC4 = LTD4 >> LTE4.
  • Tissue specificity
    Widely expressed, with highest levels in the heart, placenta, spleen, peripheral blood leukocytes and adrenal gland. In lung, expressed in the interstitial macrophages, and slightly in smooth muscle cells.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CLTR2_HUMAN antibody
    • CYSLT2 antibody
    • CYSLT2R antibody
    • CYSLTR 2 antibody
    • CysLTR2 antibody
    • Cysteinyl leukotriene CysLT2 receptor antibody
    • Cysteinyl leukotriene receptor 2 antibody
    • G protein coupled receptor antibody
    • G protein coupled receptor GPCR21 antibody
    • G protein coupled receptor HG57 antibody
    • G-protein coupled receptor GPCR21 antibody
    • G-protein coupled receptor HG57 antibody
    • GPCR antibody
    • HG57 antibody
    • hGPCR21 antibody
    • HPN321 antibody
    • PSEC0146 antibody
    see all

Images

  • ab115453, at 10 µg/ml, staining CysLT2 in formalin-fixed, paraffin-embedded Human Heart tissue by Immunohistochemistry, using a biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

References

This product has been referenced in:
  • Shirasaki H  et al. Localization and up-regulation of cysteinyl leukotriene-2 receptor in human allergic nasal mucosa. Allergol Int 62:223-8 (2013). IHC ; Human . Read more (PubMed: 23524649) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Sample
Mouse Cell (mouse/human retinal cross-sections)
Specification
mouse/human retinal cross-sections
Permeabilization
No
Fixative
Paraformaldehyde
Username

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Verified customer

Submitted Jan 13 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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