Overview

  • Product nameAnti-Cystathionase antibody
    See all Cystathionase primary antibodies
  • Description
    Rabbit polyclonal to Cystathionase
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig, Drosophila melanogaster, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to N terminal amino acids 36-85 (AVVPPISLST TFKQGAPGQH SGFEYSRSGN PTRNCLEKAV AALDGAKYCL) of human Cystathionase (NP_001893)

  • Positive control
    • Transfected 293T cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab80643 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 45 kDa.Can be blocked with Cystathionase peptide (ab139491). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionCatalyzes the last step in the transsulfuration pathway from methionine to cysteine. Has broad substrate specificity. Converts cystathionine to cysteine, ammonia and 2-oxobutanoate. Converts two cysteine molecules to lanthionine and hydrogen sulfide. Can also accept homocysteine as substrate. Specificity depends on the levels of the endogenous substrates. Generates the endogenous signaling molecule hydrogen sulfide (H2S), and so contributes to the regulation of blood pressure.
  • PathwayAmino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-homocysteine and L-serine: step 2/2.
  • Involvement in diseaseDefects in CTH are the cause of cystathioninuria (CSTNU) [MIM:219500]. It is an autosomal recessive phenotype characterized by abnormal accumulation of plasma cystathionine, leading to increased urinary excretion.
  • Sequence similaritiesBelongs to the trans-sulfuration enzymes family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • CGL_HUMAN antibody
    • CTH antibody
    • cystathionase (cystathionine gamma-lyase) antibody
    • Cystathionine gamma lyase antibody
    • Cystathionine gamma-lyase antibody
    • Cysteine desulfhydrase antibody
    • Gamma cystathionase antibody
    • Gamma-cystathionase antibody
    • Homoserine deaminase antibody
    • Homoserine dehydratase antibody
    • MGC9471 antibody
    see all

Anti-Cystathionase antibody images

  • Anti-Cystathionase antibody (ab80643) at 1 µg/ml + transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 45 kDa
    Observed band size : 45 kDa

References for Anti-Cystathionase antibody (ab80643)

This product has been referenced in:
  • Wang K  et al. Dysregulation of hydrogen sulfide producing enzyme cystathionine ?-lyase contributes to maternal hypertension and placental abnormalities in preeclampsia. Circulation 127:2514-22 (2013). WB . Read more (PubMed: 23704251) »
  • Lee HJ  et al. Hydrogen Sulfide Inhibits High Glucose-induced Matrix Protein Synthesis by Activating AMP-activated Protein Kinase in Renal Epithelial Cells. J Biol Chem 287:4451-61 (2012). WB ; Rat . Read more (PubMed: 22158625) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us.

The three cystathionase antibodies we have, aside from ab80643, have all been tested for western blotting but we do not have the corresponding blocking peptides. I recommend the monoclonal antibody ab54573, which...

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Thank you very much for keeping me up to date with the performance of the antibodies. I am very happy to hear you could obtain satisfactory results from ab54573. On the other hand it is regrettable ab80643 didn’t perform accordingly. I appreciate the t...

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Thanks for your reply. We don’t have any evidence of the expression of cystathionase in HUVECs. If you have data to support the presence of the protein in the cell line, but not in high quantities, it would be better loading bigger amounts of sample pe...

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Thank you for contacting us. Unless specified on the datasheet, all the Abcam antibodies are characterized under reducing conditions. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"