Recombinant Anti-Cystatin A antibody [EPR6941] (ab128948)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6941] to Cystatin A
- Suitable for: WB, Flow Cyt (Intra)
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-Cystatin A antibody [EPR6941]
See all Cystatin A primary antibodies -
Description
Rabbit monoclonal [EPR6941] to Cystatin A -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt (Intra)more details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide within Human Cystatin A aa 50-150 (C terminal). The exact sequence is proprietary.
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Positive control
- NIH 3T3, A549, A431, K562 cell lysates; fetal brain, fetal heart, fetal kidney and Human spleen tissue lysates; NIH3T3 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR6941 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab128948 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 13 kDa (predicted molecular weight: 11 kDa).
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 13 kDa (predicted molecular weight: 11 kDa). |
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
This is an intracellular thiol proteinase inhibitor. -
Sequence similarities
Belongs to the cystatin family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 1475 Human
- Entrez Gene: 209294 Mouse
- Entrez Gene: 288075 Rat
- Omim: 184600 Human
- SwissProt: P01040 Human
- SwissProt: P56567 Mouse
- SwissProt: P01039 Rat
- Unigene: 518198 Human
see all -
Alternative names
- AREI antibody
- cstA antibody
- Cystatin A antibody
see all
Images
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All lanes : Anti-Cystatin A antibody [EPR6941] (ab128948) at 1/1000 dilution
Lane 1 : NIH 3T3 cell lysate
Lane 2 : A549 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : K562 cell lysate
Lane 5 : Fetal brain tissue lysate
Lane 6 : Fetal heart tissue lysate
Lane 7 : Fetal kidney tissue lysate
Lane 8 : Human spleen tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 11 kDa
Observed band size: 13 kDa why is the actual band size different from the predicted? -
ab128948 at a 1/10 dilution staining Cystatin A in permeabilized NIH 3T3 cells by intracellular flow cytometry (red), with Rabbit IgG control (green).
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab128948 has been referenced in 1 publication.
- Komura T et al. Clinical features of cystatin A expression in patients with pancreatic ductal adenocarcinoma. Cancer Sci 108:2122-2129 (2017). IHC ; Human . PubMed: 28898495