Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cystatin A antibody [WR-23/2/3/3] (ab10442)
IHC image of ab10442 staining in normal human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab10442, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-Cystatin A antibody [WR-23/2/3/3] (ab10442)
This product has been referenced in:
Lundberg KC et al. Proteomics of skin proteins in psoriasis: from discovery and verification in a mouse model to confirmation in humans. Mol Cell Proteomics14:109-19 (2015).
Read more (PubMed: 25351201) »
Chang KP et al. Identification of candidate nasopharyngeal carcinoma serum biomarkers by cancer cell secretome and tissue transcriptome analysis: Potential usage of cystatin A for predicting nodal stage and poor prognosis. Proteomics10:2644-60 (2010).
Read more (PubMed: 20461718) »