Overview

  • Product name
  • Description
    Rabbit polyclonal to Cystatin C
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rhesus monkey
  • Immunogen

    Recombinant fragment within Human Cystatin C (internal sequence). The exact sequence is proprietary.
    Database link: P01034

  • Positive control
    • IHC-P: Human ovary tissue. WB: A431 and HepG2 whole cell extracts; Human plasma.

Properties

Applications

Our Abpromise guarantee covers the use of ab226841 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 16 kDa.
IHC-P 1/100 - 1/1000.

Target

  • Function
    As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.
  • Tissue specificity
    Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in various body fluids, such as the cerebrospinal fluid and plasma. Expressed in highest levels in the epididymis, vas deferens, brain, thymus, and ovary and the lowest in the submandibular gland.
  • Involvement in disease
    Amyloidosis 6
    Macular degeneration, age-related, 11
  • Sequence similarities
    Belongs to the cystatin family.
  • Post-translational
    modifications
    The Thr-25 variant is O-glycosylated with a core 1 or possibly core 8 glycan. The signal peptide of the O-glycosylated Thr-25 variant is cleaved between Ala-20 and Val-21.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • AD 8 antibody
    • AD8 antibody
    • Amyloid angiopathy and cerebral hemorrhage antibody
    • ARMD11 antibody
    • bA218C14.4 (cystatin C) antibody
    • bA218C14.4 antibody
    • Cst 3 antibody
    • Cst3 antibody
    • CST3 protein antibody
    • Cystatin 3 antibody
    • Cystatin-3 antibody
    • Cystatin-C antibody
    • Cystatin3 antibody
    • CystatinC antibody
    • CYTC_HUMAN antibody
    • Epididymis secretory protein Li 2 antibody
    • Gamma trace antibody
    • Gamma-trace antibody
    • HCCAA antibody
    • HEL S 2 antibody
    • MGC117328 antibody
    • Neuroendocrine basic polypeptide antibody
    • Post gamma globulin antibody
    • Post-gamma-globulin antibody
    see all

Images

  • Paraffin-embedded human ovary tissue stained for Cystatin C using ab226841 at 1/400 dilution in immunohistochemical analysis.

  • All lanes : Anti-Cystatin C antibody (ab226841) at 1/1000 dilution

    Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 16 kDa

    15% SDS-PAGE gel.

  • Anti-Cystatin C antibody (ab226841) at 1/1000 dilution + Human plasma at 30 µg

    Predicted band size : 16 kDa

    15% SDS-PAGE gel.

References

ab226841 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab226841.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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