Anti-Cystatin C antibody [Cyst-13] (ab24327)

Abreviews (3)Q&A (1)Specific References (2)
Customer reviews and FAQs

Overview

  • Product name
    Anti-Cystatin C antibody [Cyst-13]
    See all Cystatin C primary antibodies
  • Description
    Mouse monoclonal [Cyst-13] to Cystatin C
  • Tested applications
    Suitable for: Sandwich ELISA, ELISA, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Cystatin C protein (Human) conjugated to BSA.

Properties

Applications

Our Abpromise guarantee covers the use of ab24327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use a concentration of 1 µg/ml. For sandwich ELISA, use this antibody as Capture at 1µg/ml with ab85790 as Detection.
ELISA Use at an assay dependent dilution.
ICC/IF Use a concentration of 1 µg/ml.
WB 1/1000.

Target

  • Function
    As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.
  • Tissue specificity
    Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in various body fluids, such as the cerebrospinal fluid and plasma. Expressed in highest levels in the epididymis, vas deferens, brain, thymus, and ovary and the lowest in the submandibular gland.
  • Involvement in disease
    Amyloidosis 6
    Macular degeneration, age-related, 11
  • Sequence similarities
    Belongs to the cystatin family.
  • Post-translational
    modifications
    The Thr-25 variant is O-glycosylated with a core 1 or possibly core 8 glycan. The signal peptide of the O-glycosylated Thr-25 variant is cleaved between Ala-20 and Val-21.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
    • Alternative names
      • AD 8 antibody
      • AD8 antibody
      • Amyloid angiopathy and cerebral hemorrhage antibody
      • ARMD11 antibody
      • bA218C14.4 (cystatin C) antibody
      • bA218C14.4 antibody
      • Cst 3 antibody
      • Cst3 antibody
      • CST3 protein antibody
      • Cystatin 3 antibody
      • Cystatin-3 antibody
      • Cystatin-C antibody
      • Cystatin3 antibody
      • CystatinC antibody
      • CYTC_HUMAN antibody
      • Epididymis secretory protein Li 2 antibody
      • Gamma trace antibody
      • Gamma-trace antibody
      • HCCAA antibody
      • HEL S 2 antibody
      • MGC117328 antibody
      • Neuroendocrine basic polypeptide antibody
      • Post gamma globulin antibody
      • Post-gamma-globulin antibody
      see all

    Anti-Cystatin C antibody [Cyst-13] images

    • Sandwich ELISA - Cystatin C antibody [Cyst-13] (ab24327)
      Sandwich ELISA - Cystatin C antibody [Cyst-13] (ab24327)
      Standard Curve for Cystatin C (Analyte: ab7653) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [Cyst-13] to Cystatin C (ab24327) at 1ug/ml and Detector Antibody Rabbit polyclonal to Cystatin C (ab85790) at 0.5ug/ml
    • Immunocytochemistry/ Immunofluorescence - Cystatin C antibody [Cyst-13] (ab24327)
      Immunocytochemistry/ Immunofluorescence - Cystatin C antibody [Cyst-13] (ab24327)
      ICC/IF image of ab24327 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24327, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    References for Anti-Cystatin C antibody [Cyst-13] (ab24327)

    This product has been referenced in:
    • Zhang Y  et al. Platelet-derived miR-92a downregulates cysteine protease inhibitor cystatin C in type II diabetic lower limb ischemia. Exp Ther Med 9:2257-2262 (2015). WB . Read more (PubMed: 26136970) »
    • Schlisser AE & Hales BF Deprenyl enhances the teratogenicity of hydroxyurea in organogenesis stage mouse embryos. Toxicol Sci 134:391-9 (2013). Read more (PubMed: 23696560) »

    See all 2 Publications for this product

    Publishing research using ab24327? Please let us know so that we can cite the reference in this datasheet.

    Product Wall

    Submit an abreview for ab24327 Submit a question for ab24327

    4 Abreviews or Q&A

    10
    Votes: Highest First

    Immunohistochemistry (Frozen sections) abreview for Anti-Cystatin C antibody [Cyst-13]

     Excellent
    Abreviews
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Permeabilization
    Yes - 0.1 M PBS with 3% Triton X
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Fixative
    Paraformaldehyde
    Read More
    abreview image
    Username

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted Aug 20 2015

    HI see on your website that Abcam recommends the use of the Anti-Cystatin C antibody [Cyst-13] (ab24327) with (ab85790) for sandwich ELISA protocols. Which Abcam Cystatin C target protein would you recommend for the sandwich ELISA?

    Thank you for contacting Abcam.

    We originally tested both ab24327 & ab85790 in sandwich ELISA using our Cystain C protein, ab7653 which is unfortunately no longer available in our catalogue.

    As an alternative, I would suggest ab87...

    Read More

    Western blot abreview for Anti-Cystatin C antibody [Cyst-13]

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Glioblastoma)
    Loading amount
    40 µg
    Specification
    Glioblastoma
    Gel Running Conditions
    Reduced Denaturing (15)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 3% · Temperature: RT°C
    Read More
    abreview image
    Username

    Dr. Ratnakar Singh

    Verified customer

    Submitted Mar 29 2012

    Western blot abreview for Anti-Cystatin C antibody [Cyst-13]

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (MDA MB 231 cells)
    Loading amount
    10 µg
    Specification
    MDA MB 231 cells
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    Submitted Apr 09 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up