Anti-Cystatin C antibody [Cyst-13] (ab24327)
- Datasheet
- Specific References (2)
- Protocols
Overview
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Product nameAnti-Cystatin C antibody [Cyst-13]
See all Cystatin C primary antibodies -
DescriptionMouse monoclonal [Cyst-13] to Cystatin C
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Host speciesMouse
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Tested applicationsSuitable for: Sandwich ELISA, ELISA, ICC/IF, WBmore details
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Species reactivityReacts with: Human
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Immunogen
Full length Cystatin C protein (Human) conjugated to BSA.
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General notes
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4 -
Concentration information loading... -
PurityProtein A purified
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Purification notesPThis antibody was purified by chromatography. Purity was tested by electrophoresis.
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ClonalityMonoclonal
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Clone numberCyst-13
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MyelomaSp2/0
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IsotypeIgG1
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Research areas
Associated products
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Compatible Secondaries
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Related Products
Applications
Our Abpromise guarantee covers the use of ab24327 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use a concentration of 1 µg/ml. For sandwich ELISA, use this antibody as Capture at 1µg/ml with ab85790 as Detection. |
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| ELISA | Use at an assay dependent concentration. | |
| ICC/IF | Use a concentration of 1 µg/ml. | |
| WB | 1/1000. |
Target
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FunctionAs an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.
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Tissue specificityExpressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in various body fluids, such as the cerebrospinal fluid and plasma. Expressed in highest levels in the epididymis, vas deferens, brain, thymus, and ovary and the lowest in the submandibular gland.
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Involvement in diseaseAmyloidosis 6
Macular degeneration, age-related, 11 -
Sequence similaritiesBelongs to the cystatin family.
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Post-translational
modificationsThe Thr-25 variant is O-glycosylated with a core 1 or possibly core 8 glycan. The signal peptide of the O-glycosylated Thr-25 variant is cleaved between Ala-20 and Val-21. -
Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 1471 Human
- Omim: 604312 Human
- SwissProt: P01034 Human
- Unigene: 304682 Human
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Alternative names
- AD 8 antibody
- AD8 antibody
- Amyloid angiopathy and cerebral hemorrhage antibody
see all
Images
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![Sandwich ELISA - Anti-Cystatin C antibody [Cyst-13] (ab24327)](http://a.abcam.com/ps/products/24/ab24327/Images/Cystatin-C-Primary-antibodies-ab24327-5.jpg)
Sandwich ELISA - Anti-Cystatin C antibody [Cyst-13] (ab24327)Standard Curve for Cystatin C (Analyte: ab7653) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [Cyst-13] to Cystatin C (ab24327) at 1ug/ml and Detector Antibody Rabbit polyclonal to Cystatin C (ab85790) at 0.5ug/ml -
![Immunocytochemistry/ Immunofluorescence - Anti-Cystatin C antibody [Cyst-13] (ab24327)](http://a.abcam.com/ps/products/24/ab24327/Images/Cystatin-C-Primary-antibodies-ab24327-2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Cystatin C antibody [Cyst-13] (ab24327)ICC/IF image of ab24327 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24327, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
References
This product has been referenced in:
- Zhang Y et al. Platelet-derived miR-92a downregulates cysteine protease inhibitor cystatin C in type II diabetic lower limb ischemia. Exp Ther Med 9:2257-2262 (2015). WB . Read more (PubMed: 26136970) »
- Schlisser AE & Hales BF Deprenyl enhances the teratogenicity of hydroxyurea in organogenesis stage mouse embryos. Toxicol Sci 134:391-9 (2013). Read more (PubMed: 23696560) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"