Validated using a knockout cell line
Recombinant
RabMAb

Anti-Cystatin C antibody [EPR4413] (ab109508)

Overview

  • Product name
    Anti-Cystatin C antibody [EPR4413]
    See all Cystatin C primary antibodies
  • Description
    Rabbit monoclonal [EPR4413] to Cystatin C
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant protein fragment corresponding to residues in Human Cystatin C.

  • Positive control
    • WB: Human testis, Caco2, U20S, HeLa, HepG2, and U87-MG lysates. IHC-P: Human brain tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab109508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 13 kDa (predicted molecular weight: 15 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function
      As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.
    • Tissue specificity
      Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in various body fluids, such as the cerebrospinal fluid and plasma. Expressed in highest levels in the epididymis, vas deferens, brain, thymus, and ovary and the lowest in the submandibular gland.
    • Involvement in disease
      Amyloidosis 6
      Macular degeneration, age-related, 11
    • Sequence similarities
      Belongs to the cystatin family.
    • Post-translational
      modifications
      The Thr-25 variant is O-glycosylated with a core 1 or possibly core 8 glycan. The signal peptide of the O-glycosylated Thr-25 variant is cleaved between Ala-20 and Val-21.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • AD 8 antibody
      • AD8 antibody
      • Amyloid angiopathy and cerebral hemorrhage antibody
      • ARMD11 antibody
      • bA218C14.4 (cystatin C) antibody
      • bA218C14.4 antibody
      • Cst 3 antibody
      • Cst3 antibody
      • CST3 protein antibody
      • Cystatin 3 antibody
      • Cystatin-3 antibody
      • Cystatin-C antibody
      • Cystatin3 antibody
      • CystatinC antibody
      • CYTC_HUMAN antibody
      • Epididymis secretory protein Li 2 antibody
      • Gamma trace antibody
      • Gamma-trace antibody
      • HCCAA antibody
      • HEL S 2 antibody
      • MGC117328 antibody
      • Neuroendocrine basic polypeptide antibody
      • Post gamma globulin antibody
      • Post-gamma-globulin antibody
      see all

    Anti-Cystatin C antibody [EPR4413] images



    • Predicted band size : 15 kDa

      Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: CST3 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: Caco2 whole cell lysate (20 µg)
      Lane 4: U20S whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab109508 observed at 15 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab109508 was shown to specifically react with CST3 when CST3 knockout samples were used. Wild-type and CST3 knockout samples were subjected to SDS-PAGE. Ab109508 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 10000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-Cystatin C antibody [EPR4413] (ab109508) at 1/10000 dilution

      Lane 1 : Human testis lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : U87-MG cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 15 kDa
      Observed band size : 13 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of paraffin-embedded Human brain tissue using ab109508 at adilution of 1/250.

    References for Anti-Cystatin C antibody [EPR4413] (ab109508)

    This product has been referenced in:
    • Furukawa S  et al. Databases for technical aspects of immunohistochemistry. J Toxicol Pathol 30:79-107 (2017). IHC ; Rat . Read more (PubMed: 28190929) »
    • Wang T  et al. Lycium barbarum polysaccharide prevents focal cerebral ischemic injury by inhibiting neuronal apoptosis in mice. PLoS One 9:e90780 (2014). WB, IHC-P ; Mouse . Read more (PubMed: 24595452) »

    See all 3 Publications for this product

    Product Wall

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Permeabilization
    Yes - 0.1 M PBS with 3% Triton X
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Fixative
    Paraformaldehyde
    Username

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted Aug 20 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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