• Product nameAnti-Cystatin C antibody [EPR4413]
    See all Cystatin C primary antibodies
  • Description
    Rabbit monoclonal [EPR4413] to Cystatin C
  • Tested applicationsSuitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant protein fragment corresponding to residues in Human Cystatin C.

  • Positive control
    • Human testis, HeLa, HepG2, and U87-MG lysates, Human brain tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.



Our Abpromise guarantee covers the use of ab109508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 13 kDa (predicted molecular weight: 15 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notesIs unsuitable for Flow Cyt,ICC or IP.
  • Target

    • FunctionAs an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.
    • Tissue specificityExpressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in various body fluids, such as the cerebrospinal fluid and plasma. Expressed in highest levels in the epididymis, vas deferens, brain, thymus, and ovary and the lowest in the submandibular gland.
    • Involvement in diseaseAmyloidosis 6
      Macular degeneration, age-related, 11
    • Sequence similaritiesBelongs to the cystatin family.
    • Post-translational
      The Thr-25 variant is O-glycosylated with a core 1 or possibly core 8 glycan. The signal peptide of the O-glycosylated Thr-25 variant is cleaved between Ala-20 and Val-21.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • AD 8 antibody
      • AD8 antibody
      • Amyloid angiopathy and cerebral hemorrhage antibody
      • ARMD11 antibody
      • bA218C14.4 (cystatin C) antibody
      • bA218C14.4 antibody
      • Cst 3 antibody
      • Cst3 antibody
      • CST3 protein antibody
      • Cystatin 3 antibody
      • Cystatin-3 antibody
      • Cystatin-C antibody
      • Cystatin3 antibody
      • CystatinC antibody
      • CYTC_HUMAN antibody
      • Gamma trace antibody
      • Gamma-trace antibody
      • HCCAA antibody
      • MGC117328 antibody
      • Neuroendocrine basic polypeptide antibody
      • Post gamma globulin antibody
      • Post-gamma-globulin antibody
      see all

    Anti-Cystatin C antibody [EPR4413] images

    • All lanes : Anti-Cystatin C antibody [EPR4413] (ab109508) at 1/10000 dilution

      Lane 1 : Human testis lysate
      Lane 2 : HeLa testis lysate
      Lane 3 : HepG2 testis lysate
      Lane 4 : U87-MG testis lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 15 kDa
      Observed band size : 13 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of paraffin-embedded Human brain tissue using ab109508 at adilution of 1/250.

    References for Anti-Cystatin C antibody [EPR4413] (ab109508)

    This product has been referenced in:
    • Wang T  et al. Lycium barbarum polysaccharide prevents focal cerebral ischemic injury by inhibiting neuronal apoptosis in mice. PLoS One 9:e90780 (2014). WB, IHC-P ; Mouse . Read more (PubMed: 24595452) »
    • Spanu S  et al. Urinary exosomes: a novel means to non-invasively assess changes in renal gene and protein expression. PLoS One 9:e109631 (2014). IHC ; Rat . Read more (PubMed: 25310591) »

    See all 2 Publications for this product

    Product Wall

    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (Brain)
    Permeabilization Yes - 0.1 M PBS with 3% Triton X
    Specification Brain
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Fixative Paraformaldehyde

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted Aug 20 2015