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Products:Cell Biology >> Proteolysis / Ubiquitin >> Protease inhibitors >> Other protease inhibitors
Overview
Product name
Anti-Cystatin SN antibody
See all Cystatin SN products (2) ...
Description
Mouse polyclonal to Cystatin SN
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Cystatin SN (NP_001889.2, 1 a.a. ~ 141 a.a) full length human protein.
Positive control
Cystatin SN transfected 293T cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Protease inhibitors >> Other protease inhibitors
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Western blot - Cystatin SN antibody (ab68329)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab68329 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 13 kDa (predicted molecular weight: 16 kDa).
Target
Function
Human saliva appears to contain several cysteine proteinase inhibitors that are immunologically related to cystatin S but that differ in their specificity due to amino acid sequence differences. Cystatin SN, with a pI of 7.5, is a much better inhibitor of papain and dipeptidyl peptidase I than is cystatin S, although both inhibit ficin equally well.
Tissue specificity
Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in saliva, tears, urine and seminal fluid.
Sequence similarities
Belongs to the cystatin family.
Cellular localization
Secreted.
Target information above from: UniProt accessionP01037
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CST1 antibody
- Cystain SAI antibody
- Cystain-SA-I antibody
see all
Database links
- Entrez Gene: 1469 Human
- Omim: 123855 Human
- SwissProt: P01037 Human
- Unigene: 123114 Human
Anti-Cystatin SN antibody images:
Western blot - Cystatin SN antibody (ab68329)
All lanes : Anti-Cystatin SN antibody (ab68329) at 1/500 dilution
Lane 1 : Cystatin SN transfected 293T cell lysate
Lane 2 : non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 16 kDa
Observed band size : 13 kDa (why is the actual band size different from the predicted?)
References for Anti-Cystatin SN antibody (ab68329)
ab68329 has not yet been referenced specifically in any publications.
Publishing research using ab68329? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"