Anti-Cytochrome b-c1 complex subunit 9 antibody (ab84901)

Overview

  • Product name
    Anti-Cytochrome b-c1 complex subunit 9 antibody
    See all Cytochrome b-c1 complex subunit 9 primary antibodies
  • Description
    Rabbit polyclonal to Cytochrome b-c1 complex subunit 9
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within the internal sequence amino acids 14-63 (LFRRTSTFAL TIIVGVMFFE RAFDQGADAI YDHINEGKLW KHIKHKYENK) of human Cytochrome b-c1 complex subunit 9 (NP_037519).

  • Positive control
    • MCF7 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab84901 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 7 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P 1/100.

Target

  • Relevance
    Function: This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. This subunit interacts with cytochrome c1. Similarity: Belongs to the UQCR10/QCR9 family.
  • Cellular localization
    Mitochondrial; Mitochondrion inner membrane
  • Database links
  • Alternative names
    • Cytochrome c1 non heme 7 kDa protein antibody
    • Cytochrome C1 nonheme 7kDa protein antibody
    • HSPC051 antibody
    • HSPC119 antibody
    • HSPC151 antibody
    • QCR9 antibody
    • Ubiquinol cytochrome c reductase complex antibody
    • Ubiquinol cytochrome c reductase complex (7.2 kD) antibody
    • Ubiquinol cytochrome c reductase complex 7.2 kDa protein antibody
    • Ubiquinol cytochrome c reductase complex III subunit X 7.2kDa antibody
    • Ubiquinol cytochrome c reductase complex III subunit X antibody
    • UCCR7.2 antibody
    • UCRC antibody
    • UQCR10 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bronchial epithelial tissue labelling Cytochrome b-c1 complex subunit 9 with ab84901 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - Cytochrome b-c1 complex subunit 9. Right - Merge.
  • Anti-Cytochrome b-c1 complex subunit 9 antibody (ab84901) at 1 µg/ml (in 5% skim milk / PBS buffer) + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 7 kDa
    Observed band size : <10 kDa (why is the actual band size different from the predicted?)
    Gel concentration: 10-20%

References

This product has been referenced in:
  • Li L  et al. Proteomic analysis of differentially expressed proteins in kidneys of brain dead rabbits. Mol Med Rep 16:215-223 (2017). WB ; Rabbit . Read more (PubMed: 28534953) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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