Human Predicted to work with:
Mouse, Rat, Rabbit, Guinea pig, Cow, Cat, Dog, Pig
Synthetic peptide corresponding to a region within the internal sequence amino acids 14-63 (LFRRTSTFAL TIIVGVMFFE RAFDQGADAI YDHINEGKLW KHIKHKYENK) of human Cytochrome b-c1 complex subunit 9 (NP_037519).
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 7 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
RelevanceFunction: This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. This subunit interacts with cytochrome c1.
Similarity: Belongs to the UQCR10/QCR9 family.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bronchial epithelial tissue labelling Cytochrome b-c1 complex subunit 9 with ab84901 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - Cytochrome b-c1 complex subunit 9. Right - Merge.
Western blot - Cytochrome b-c1 complex subunit 9 antibody (ab84901)
Anti-Cytochrome b-c1 complex subunit 9 antibody (ab84901) at 1 µg/ml (in 5% skim milk / PBS buffer) + MCF7 cell lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution