Recombinant Anti-Cytochrome b-c1 complex subunit 9/UQCR10 antibody [EPR8179] (ab134909)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8179] to Cytochrome b-c1 complex subunit 9/UQCR10
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Cytochrome b-c1 complex subunit 9/UQCR10 antibody [EPR8179]
See all Cytochrome b-c1 complex subunit 9/UQCR10 primary antibodies -
Description
Rabbit monoclonal [EPR8179] to Cytochrome b-c1 complex subunit 9/UQCR10 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Cytochrome b-c1 complex subunit 9/UQCR10 aa 50-150. The exact sequence is proprietary.
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Positive control
- MCF7, HepG2, HeLa, Human fetal heart, and Human skeletal muscle lysates; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Dissociation constant (KD)
KD = 3.40 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8179 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134909 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000 - 1/50000. Predicted molecular weight: 7 kDa.
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ICC/IF |
1/250 - 1/500.
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Notes |
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WB
1/10000 - 1/50000. Predicted molecular weight: 7 kDa. |
ICC/IF
1/250 - 1/500. |
Target
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Relevance
Function: This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. This subunit interacts with cytochrome c1. Similarity: Belongs to the UQCR10/QCR9 family. -
Cellular localization
Mitochondrial; Mitochondrion inner membrane -
Database links
- Entrez Gene: 29796 Human
- Omim: 610843 Human
- SwissProt: Q9UDW1 Human
- Unigene: 284292 Human
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Alternative names
- Cytochrome c1 non heme 7 kDa protein antibody
- Cytochrome C1 nonheme 7kDa protein antibody
- HSPC051 antibody
see all
Images
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All lanes : Anti-Cytochrome b-c1 complex subunit 9/UQCR10 antibody [EPR8179] (ab134909) at 1/10000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Human fetal heart tissue lysate
Lane 5 : Human skeletal muscle lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG polyclonal at 1/2000 dilution
Predicted band size: 7 kDa -
Immunofluorescent analysis of HeLa cells labelling Cytochrome b-c1 complex subunit 9/UQCR10 with ab134909 at 1/250 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab134909 has been referenced in 1 publication.
- Daniel HD & Torbenson M Transgenic hepatitis B: a new model of HBV infection. Sci Rep 7:2610 (2017). PubMed: 28572637