Anti-Cytochrome b reductase 1 antibody (ab28758)

Overview

  • Product nameAnti-Cytochrome b reductase 1 antibody
    See all Cytochrome b reductase 1 primary antibodies
  • Description
    Goat polyclonal to Cytochrome b reductase 1
  • Tested applicationsSuitable for: ICC/IF, IHC-Fr, IHC-FoFr, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Cow, Dog, Pig
  • Immunogen

    Synthetic peptide:

    (C)RNLALDEAGQRSTM

    , corresponding to C terminal amino acids 273-286 of Human Cytochrome b reductase 1

Properties

Applications

Our Abpromise guarantee covers the use of ab28758 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.
IHC-Fr Use at an assay dependent concentration.
IHC-FoFr 1/200.
IHC-P Use a concentration of 2 - 5 µg/ml.

Target

  • FunctionFerric-chelate reductase that reduces Fe(3+) to Fe(2+). Present at the brush border of duodenal enterocytes where it probably reduces dietary Fe(3+) thereby facilitating its transport into the mucosal cells. Uses ascorbate as electron donor. May be involved in extracellular ascorbate recycling in erythrocyte membranes. May also act as a ferrireductase in airway epithelial cells.
  • Tissue specificityPresent in erythrocyte membranes (at protein level). Also expressed in respiratory epithelium.
  • Sequence similaritiesContains 1 cytochrome b561 domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CYBR 1 antibody
    • CYBR1 antibody
    • CYBR1_HUMAN antibody
    • CYBRD 1 antibody
    • cybrd1 antibody
    • Cytochrome b reductase 1 antibody
    • DCYTB antibody
    • DKFZp564E227 antibody
    • Duodenal cytochrome b antibody
    • Ferric chelate reductase 3 antibody
    • Ferric-chelate reductase 3 antibody
    • FLJ23462 antibody
    • FRRS3 antibody
    see all

Anti-Cytochrome b reductase 1 antibody images

  • ICC/IF image of adult mouse gut section stained with (ab28758) . Tissue was perfusion fixed with 4% PFA. The sections were incubated in 10% normal donkey serum in 0.1% PBS- and triton X100 for 1h to permeabilise the cells and block non-specific protein-protein interactions. The sections were then incubated with the antibody (ab28758) @ 1µg/ml) overnight @ 4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    See Abreview

  • ab28758 at 2.5 µg/ml staining Cytochrome b reductase 1 in Human testis by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
  • ICC/IF image of Cytochrome b reductase staining on Caco 2 cells using ab28758 (1:200). The cells were fixed using 4% PFA and permeabilized using 0.1% TritonX in 0.1% PBS. The cells were blocked by incubating 10% donkey serum for 1 hour at 24°C. ab28758 (1:200) was incubated for 4 hours at 24°C. the secondary antibody used was donkey polyclonal to Goat IgG conjugated to Alexa Fluor 568.

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References for Anti-Cytochrome b reductase 1 antibody (ab28758)

ab28758 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Human duodenal cell line)
Specification Human duodenal cell line
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% TritonX in 0.1% PBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Feb 18 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Duodenum)
Specification Duodenum
Fixative Paraformaldehyde
Permeabilization Yes - PBS-0.3x Triton X
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Jun 21 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"