Anti-Cytochrome b reductase 1 antibody (ab28758)

Overview

  • Product name
    Anti-Cytochrome b reductase 1 antibody
    See all Cytochrome b reductase 1 primary antibodies
  • Description
    Goat polyclonal to Cytochrome b reductase 1
  • Tested applications
    Suitable for: ICC/IF, IHC-Fr, IHC-FoFr, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Cow, Dog, Pig
  • Immunogen

    Synthetic peptide:

    (C)RNLALDEAGQRSTM

    , corresponding to C terminal amino acids 273-286 of Human Cytochrome b reductase 1

Properties

Applications

Our Abpromise guarantee covers the use of ab28758 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.
IHC-Fr Use at an assay dependent concentration.
IHC-FoFr 1/200.
IHC-P Use a concentration of 2 - 5 µg/ml.

Target

  • Function
    Ferric-chelate reductase that reduces Fe(3+) to Fe(2+). Present at the brush border of duodenal enterocytes where it probably reduces dietary Fe(3+) thereby facilitating its transport into the mucosal cells. Uses ascorbate as electron donor. May be involved in extracellular ascorbate recycling in erythrocyte membranes. May also act as a ferrireductase in airway epithelial cells.
  • Tissue specificity
    Present in erythrocyte membranes (at protein level). Also expressed in respiratory epithelium.
  • Sequence similarities
    Contains 1 cytochrome b561 domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CYBR 1 antibody
    • CYBR1 antibody
    • CYBR1_HUMAN antibody
    • CYBRD 1 antibody
    • cybrd1 antibody
    • Cytochrome b reductase 1 antibody
    • DCYTB antibody
    • DKFZp564E227 antibody
    • Duodenal cytochrome b antibody
    • Ferric chelate reductase 3 antibody
    • Ferric-chelate reductase 3 antibody
    • FLJ23462 antibody
    • FRRS3 antibody
    see all

Images

  • ICC/IF image of adult mouse gut section stained with (ab28758) . Tissue was perfusion fixed with 4% PFA. The sections were incubated in 10% normal donkey serum in 0.1% PBS- and triton X100 for 1h to permeabilise the cells and block non-specific protein-protein interactions. The sections were then incubated with the antibody (ab28758) @ 1µg/ml) overnight @ 4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    See Abreview

  • ab28758 at 2.5 µg/ml staining Cytochrome b reductase 1 in Human testis by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
  • ICC/IF image of Cytochrome b reductase staining on Caco 2 cells using ab28758 (1:200). The cells were fixed using 4% PFA and permeabilized using 0.1% TritonX in 0.1% PBS. The cells were blocked by incubating 10% donkey serum for 1 hour at 24°C. ab28758 (1:200) was incubated for 4 hours at 24°C. the secondary antibody used was donkey polyclonal to Goat IgG conjugated to Alexa Fluor 568.

    See Abreview

References

ab28758 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human duodenal cell line)
Specification
Human duodenal cell line
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% TritonX in 0.1% PBS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Feb 18 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Duodenum)
Specification
Duodenum
Fixative
Paraformaldehyde
Permeabilization
Yes - PBS-0.3x Triton X
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Jun 21 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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