Anti-Cytochrome b reductase 1 antibody (ab66048)

Overview

  • Product nameAnti-Cytochrome b reductase 1 antibody
    See all Cytochrome b reductase 1 primary antibodies
  • Description
    Rabbit polyclonal to Cytochrome b reductase 1
  • Tested applicationsSuitable for: ICC/IF, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Pig, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Mouse Cytochrome b reductase 1.

    Peptide available as ab95410

  • Positive control
    • This antibody gave a positive signal in A20 whole cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab66048 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
IHC-Fr Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).

Target

  • FunctionFerric-chelate reductase that reduces Fe(3+) to Fe(2+). Present at the brush border of duodenal enterocytes where it probably reduces dietary Fe(3+) thereby facilitating its transport into the mucosal cells. Uses ascorbate as electron donor. May be involved in extracellular ascorbate recycling in erythrocyte membranes. May also act as a ferrireductase in airway epithelial cells.
  • Tissue specificityPresent in erythrocyte membranes (at protein level). Also expressed in respiratory epithelium.
  • Sequence similaritiesContains 1 cytochrome b561 domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CYBR 1 antibody
    • CYBR1 antibody
    • CYBR1_HUMAN antibody
    • CYBRD 1 antibody
    • cybrd1 antibody
    • Cytochrome b reductase 1 antibody
    • DCYTB antibody
    • DKFZp564E227 antibody
    • Duodenal cytochrome b antibody
    • Ferric chelate reductase 3 antibody
    • Ferric-chelate reductase 3 antibody
    • FLJ23462 antibody
    • FRRS3 antibody
    see all

Anti-Cytochrome b reductase 1 antibody images

  • Anti-Cytochrome b reductase 1 antibody (ab66048) at 1 µg/ml + A20 whole cell lysate (ab7180) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 31 kDa
    Observed band size : 31 kDa


    Exposure time : 10 minutes
  • IHC-Fr image of human duodenum section stained with ab66048. The sections were incubated in 10% normal donkey serum in 0.1% PBS- and 0.3x triton100 for 1h to permeabilise the cells and to block non-specific protein-protein interactions. The sections were then incubated with the antibody ab66048, 1µg/ml) and Anti-Actin overnight at +4°C. The secondary antibody was Alexa Alexa Fluor® 488 goat anti-rabbit IgG (H+L) and Fluor®568 donkey anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. dCytb stained apical site of duodenum and actin stained muscles and blood vessels.

    See Abreview

  • ICC/IF image of ab66048 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66048, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Cytochrome b reductase 1 antibody (ab66048)

ab66048 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Astrocytes culture)
Specification Astrocytes culture
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% TritonX in 0.1% PBS
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Mar 22 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Duodenum)
Specification Duodenum
Fixative Paraformaldehyde
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Sep 06 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"