Anti-Cytochrome b reductase 1 antibody (ab66048)

Overview

  • Product nameAnti-Cytochrome b reductase 1 antibody
    See all Cytochrome b reductase 1 primary antibodies
  • Description
    Rabbit polyclonal to Cytochrome b reductase 1
  • Tested applicationsSuitable for: ICC/IF, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Pig, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Mouse Cytochrome b reductase 1.

    Peptide available as ab95410

  • Positive control
    • This antibody gave a positive signal in A20 whole cell lysate. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab66048 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
IHC-Fr Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).

Target

  • FunctionFerric-chelate reductase that reduces Fe(3+) to Fe(2+). Present at the brush border of duodenal enterocytes where it probably reduces dietary Fe(3+) thereby facilitating its transport into the mucosal cells. Uses ascorbate as electron donor. May be involved in extracellular ascorbate recycling in erythrocyte membranes. May also act as a ferrireductase in airway epithelial cells.
  • Tissue specificityPresent in erythrocyte membranes (at protein level). Also expressed in respiratory epithelium.
  • Sequence similaritiesContains 1 cytochrome b561 domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CYBR 1 antibody
    • CYBR1 antibody
    • CYBR1_HUMAN antibody
    • CYBRD 1 antibody
    • cybrd1 antibody
    • Cytochrome b reductase 1 antibody
    • DCYTB antibody
    • DKFZp564E227 antibody
    • Duodenal cytochrome b antibody
    • Ferric chelate reductase 3 antibody
    • Ferric-chelate reductase 3 antibody
    • FLJ23462 antibody
    • FRRS3 antibody
    see all

Anti-Cytochrome b reductase 1 antibody images

  • Anti-Cytochrome b reductase 1 antibody (ab66048) at 1 µg/ml + A20 whole cell lysate (ab7180) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 31 kDa
    Observed band size : 31 kDa


    Exposure time : 10 minutes
  • IHC-Fr image of human duodenum section stained with ab66048. The sections were incubated in 10% normal donkey serum in 0.1% PBS- and 0.3x triton100 for 1h to permeabilise the cells and to block non-specific protein-protein interactions. The sections were then incubated with the antibody ab66048, 1µg/ml) and Anti-Actin overnight at +4°C. The secondary antibody was Alexa Alexa Fluor® 488 goat anti-rabbit IgG (H+L) and Fluor®568 donkey anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. dCytb stained apical site of duodenum and actin stained muscles and blood vessels.

    See Abreview

  • ICC/IF image of ab66048 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66048, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Cytochrome b reductase 1 antibody (ab66048)

ab66048 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Astrocytes culture)
Specification Astrocytes culture
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% TritonX in 0.1% PBS
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Mar 22 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Duodenum)
Specification Duodenum
Fixative Paraformaldehyde
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Sep 06 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"