The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: Use at an assay dependent dilution.
WB: 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 15 kDa).
This antibody has only been tested against the recombinant fragment, and not the endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionCytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases.
Involvement in diseaseDefects in CYB5A are the cause of methemoglobinemia CYB5A-related (METHB-CYB5A) [MIM:250790]. A form of methemoglobinemia, a hematologic disease characterized by the presence of excessive amounts of methemoglobin in blood cells, resulting in decreased oxygen carrying capacity of the blood, cyanosis and hypoxia.
Sequence similaritiesBelongs to the cytochrome b5 family. Contains 1 cytochrome b5 heme-binding domain.
Cellular localizationCytoplasm and Endoplasmic reticulum membrane. Microsome membrane.