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Bovine heart Cytochrome C
Our Abpromise guarantee covers the use of ab110325 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.25 - 1 µg/ml. Predicted molecular weight: 12 kDa.|
|ICC/IF||Use a concentration of 0.5 - 2 µg/ml. Heat induced antigen retrieval in 0.1 M Tris-HCl, 5% urea, pH 9.5 for 5 min at 95°C improves signal.|
|Flow Cyt||Use a concentration of 1 µg/ml. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.|
|In-Cell ELISA||Use a concentration of 4 µg/ml.|
ab110325 staining Cytochrome C in MDA MB 231 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 1% Triton X-100 and blocked with 10% BSA for 1 hour at 21°C. Samples were incubated with primary antibody (1/100) for 1 hour. An undiluted DyLight® 550-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.