Overview

  • Product name
    Anti-Cytochrome C antibody
    See all Cytochrome C primary antibodies
  • Description
    Rabbit polyclonal to Cytochrome C
  • Specificity
    This antibody detects the 15 kDa cytochrome C.
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken
  • Immunogen

    Synthetic peptide corresponding to residues surrounding amino acid 70 of rat Cytochrome C. (Peptide available as ab38265.)

  • Positive control
    • Jurkat cell lysate, NIH3T3 cell lysate and rat kidney tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab18738 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 12 kDa.Can be blocked with Cytochrome C peptide (ab38265).
IP Use a concentration of 10 - 20 µg/ml.

Target

  • Function
    Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.
    Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
  • Involvement in disease
    Defects in CYCS are the cause of thrombocytopenia type 4 (THC4) [MIM:612004]; also known as autosomal dominant thrombocytopenia type 4. Thrombocytopenia is the presence of relatively few platelets in blood. THC4 is a non-syndromic form of thrombocytopenia. Clinical manifestations of thrombocytopenia are absent or mild. THC4 may be caused by dysregulated platelet formation.
  • Sequence similarities
    Belongs to the cytochrome c family.
  • Post-translational
    modifications
    Binds 1 heme group per subunit.
  • Cellular localization
    Mitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • CYC antibody
    • CYC_HUMAN antibody
    • CYCS antibody
    • Cytochrome c antibody
    • Cytochrome c somatic antibody
    • HCS antibody
    • THC4 antibody
    see all

Images



  • Predicted band size : 12 kDa

    Western Blot analysis of Left Lane: Jurkat cell lysate and Right Lane: 3T3 cell lysates labeling Cytochrome C with ab18738.

References

This product has been referenced in:
  • Boyd PJ  et al. Bioenergetic status modulates motor neuron vulnerability and pathogenesis in a zebrafish model of spinal muscular atrophy. PLoS Genet 13:e1006744 (2017). WB . Read more (PubMed: 28426667) »
  • Dutta S  et al. The steroid hormone environment during primordial follicle formation in perinatal mouse ovaries. Biol Reprod 91:68 (2014). Mouse . Read more (PubMed: 25078683) »

See all 6 Publications for this product

Customer reviews and Q&As

Thank you for your enquiry. I'm happy to report that the immunogen shares 100% homology with the sequence in chicken. I hope this information helps. Please do not hesitate to contact us if you need anything further.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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