Overview

  • Product name
    Anti-Cytochrome C antibody
    See all Cytochrome C primary antibodies
  • Description
    Sheep polyclonal to Cytochrome C
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Rabbit, Dog, Human
  • Immunogen

    Full length native protein (purified) (Rabbit) conjugated to KLH

  • Positive control
    • Human heart tissue, whole extracts of MCF 7, Jurkat, Rat 1, and MDCK cells.

Applications

Our Abpromise guarantee covers the use of ab49879 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 11 kDa.
IHC-P Use a concentration of 20 - 40 µg/ml.
ICC/IF Use a concentration of 5 - 10 µg/ml.

Target

  • Function
    Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.
    Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
  • Involvement in disease
    Defects in CYCS are the cause of thrombocytopenia type 4 (THC4) [MIM:612004]; also known as autosomal dominant thrombocytopenia type 4. Thrombocytopenia is the presence of relatively few platelets in blood. THC4 is a non-syndromic form of thrombocytopenia. Clinical manifestations of thrombocytopenia are absent or mild. THC4 may be caused by dysregulated platelet formation.
  • Sequence similarities
    Belongs to the cytochrome c family.
  • Post-translational
    modifications
    Binds 1 heme group per subunit.
  • Cellular localization
    Mitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • CYC antibody
    • CYC_HUMAN antibody
    • CYCS antibody
    • Cytochrome c antibody
    • Cytochrome c somatic antibody
    • HCS antibody
    • THC4 antibody
    see all

Images

  • ab49879 at 5 µg/ml staining Human MCF-7 cells. Cells were fixed and permeabilized with 3% paraformaldehyde followed by 0.5% TritonX-100 and visualised with Rabbit anti-Sheep IgG, FITC conjugate.

References

This product has been referenced in:
  • Desmurs M  et al. C11orf83, a mitochondrial cardiolipin-binding protein involved in bc1 complex assembly and supercomplex stabilization. Mol Cell Biol 35:1139-56 (2015). Read more (PubMed: 25605331) »
  • Mu S  et al. Diosgenin induces apoptosis in IGF-1-stimulated human thyrocytes through two caspase-dependent pathways. Biochem Biophys Res Commun 418:347-52 (2012). Read more (PubMed: 22266324) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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