Overview

  • Product nameAnti-Cytochrome C antibody
    See all Cytochrome C primary antibodies
  • Description
    Sheep polyclonal to Cytochrome C
  • SpecificityThis antibody reacts specifically with cytochrome C (12.3 kDa).
  • Tested applicationsSuitable for: WB, IP, ICC/IF, ICCmore details
  • Species reactivity
    Reacts with: Rat, Rabbit, Dog, Human
  • Immunogen

    Full length native protein (purified) (Rabbit).

Applications

Our Abpromise guarantee covers the use of ab6400 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
ICC/IF
ICC
  • Application notesICC: 1/650.
    IF: Use at an assay dependant dilution.
    IP: 1 µl will immunoprecipitate cytochrome C from 0.5 mg of MCF-7 cell lysate.
    WB: 1/10000 using MCF-7, Rat-1, MDCK and Jurkat cell lysates, anti-sheep IgG conjugated to peroxidase and ECL.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionElectron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.
      Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
    • Involvement in diseaseDefects in CYCS are the cause of thrombocytopenia type 4 (THC4) [MIM:612004]; also known as autosomal dominant thrombocytopenia type 4. Thrombocytopenia is the presence of relatively few platelets in blood. THC4 is a non-syndromic form of thrombocytopenia. Clinical manifestations of thrombocytopenia are absent or mild. THC4 may be caused by dysregulated platelet formation.
    • Sequence similaritiesBelongs to the cytochrome c family.
    • Post-translational
      modifications
      Binds 1 heme group per subunit.
    • Cellular localizationMitochondrion matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • CYC antibody
      • CYC_HUMAN antibody
      • CYCS antibody
      • Cytochrome c antibody
      • Cytochrome c somatic antibody
      • HCS antibody
      • THC4 antibody
      see all

    References for Anti-Cytochrome C antibody (ab6400)

    This product has been referenced in:
    • Melli G  et al. Spatially distinct and functionally independent mechanisms of axonal degeneration in a model of HIV-associated sensory neuropathy. Brain 129:1330-8 (2006). ICC/IF ; Rat . Read more (PubMed: 16537566) »

    See 1 Publication for this product

    Product Wall

    For a secondary to follow the primary anti-caspase 3 antibody, which is raised in rabbit, I suggest ab97051, which is goat anti-rabbit IgG, conjugated to HRP. This is assuming you are using an ECL-based detection after the secondary HRP conjugate. ...

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    Thank you for contacting us. We do not have this antibody, ab6400, as a streptavidin conjugate and we do not offer conjugation service. However, we do have conjugation kits that make conjugation to avidin very simple. If you are interested, the cat...

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    Thank you for your enquiry. To clarify I can tell you that this antibody has tested on on fixed cells, not frozen tissue. A MDCK (Dog kidney) lysate was tested by western. However, canine cells are yet to be tested by immunocytochemistry. I hop...

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    Thank you for your enquiry. We do have a sheep polyclonal to cytochrome C, (ab6400) which has been tested in dog. This is raised against the whole protein, so I am sorry I cannot be more specific about the epitopes. I have found a website and a referen...

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    I am sorry you are having problems with ab6400. Thank you for taking the time to fill in our online questionnaire, it is very useful. I would like to recommend a shorter fixation time (30min) or try ice cold methanol fixation and to follow the recomme...

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    All the information we have on species cross reactivity is specified on the datasheet. To our knowledge, cross reactivity with Xenopus has not yet been tested. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Ab...

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    This antibody is supplied as whole serum and is recommended for use in WB at a dilution of 1:10,000 with whole cell lysates. Are you using whole cell extracts - from what species is your cytochrome C derived? You say that you have used the anitbody at...

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    No, I am afraid that we do not sell secondary antibodies conjugated to gold particles

    To our knowledge this antibody has not been tested on paraffin embedded specimens.

    This antibody has not been tested on murine cytochrome c, but given the conservation of this protein, we would expect it to work for mouse.

    1-10 of 12 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"