Recombinant
RabMAb

Anti-Cytochrome C antibody [EP1326-80-5-4] (ab76237)

Submit an Abreview Q&A (1)Specific References (3)

Overview

  • Product name
    Anti-Cytochrome C antibody [EP1326-80-5-4]
    See all Cytochrome C primary antibodies
  • Description
    Rabbit monoclonal [EP1326-80-5-4] to Cytochrome C
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues near the N-terminus of human Cytochrome C

  • Positive control
    • HeLa mitochondrial lysate Human heart muscle tissue Human kidney tissue
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab76237 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250 - 1/500. Detects a band of approximately 14 kDa.
IP 1/50.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Use of HRP-conjugated or polymerized HRP secondary antibodies recommended, stronger signals have been found using the polymerized HRP secondary.
ICC 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.

    • Target

    • Function
      Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.
      Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
    • Involvement in disease
      Defects in CYCS are the cause of thrombocytopenia type 4 (THC4) [MIM:612004]; also known as autosomal dominant thrombocytopenia type 4. Thrombocytopenia is the presence of relatively few platelets in blood. THC4 is a non-syndromic form of thrombocytopenia. Clinical manifestations of thrombocytopenia are absent or mild. THC4 may be caused by dysregulated platelet formation.
    • Sequence similarities
      Belongs to the cytochrome c family.
    • Post-translational
      modifications
      Binds 1 heme group per subunit.
    • Cellular localization
      Mitochondrion matrix.
    • Information by UniProt
    • Database links
      see all
    • Alternative names
      • CYC antibody
      • CYC_HUMAN antibody
      • CYCS antibody
      • Cytochrome c antibody
      • Cytochrome c somatic antibody
      • HCS antibody
      • THC4 antibody
      see all

    Images

    • Western blot - Anti-Cytochrome C antibody [EP1326-80-5-4] (ab76237)
      Western blot - Anti-Cytochrome C antibody [EP1326-80-5-4] (ab76237)
      Anti-Cytochrome C antibody [EP1326-80-5-4] (ab76237) at 1/500 dilution + HeLa cell lysate at 10 µg

      Secondary
      goat anti-rabbit HRP at 1/1000 dilution

      Observed band size: 14 kDa (why is the actual band size different from the predicted?)

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [EP1326-80-5-4] (ab76237)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [EP1326-80-5-4] (ab76237)
      ab76237, at a 1/100 dilution, staining human Cytochrome C in heart muscle by Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [EP1326-80-5-4] (ab76237)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [EP1326-80-5-4] (ab76237)
      ab76237, at a 1/100 dilution, staining human Cytochrome C in kidney by Immunohistochemistry Formalin/PFA-fixed paraffin-embedded tissue.

    References

    This product has been referenced in:
    • Bhattacharyya S  et al. Amelioration of aspirin induced oxidative impairment and apoptotic cell death by a novel antioxidant protein molecule isolated from the herb Phyllanthus niruri. PLoS One 9:e89026 (2014). Mouse . Read more (PubMed: 24586486) »
    • Wang Y  et al. Acridone derivative 8a induces oxidative stress-mediated apoptosis in CCRF-CEM leukemia cells: application of metabolomics in mechanistic studies of antitumor agents. PLoS One 8:e63572 (2013). WB ; Human . Read more (PubMed: 23667640) »

    See all 3 Publications for this product

    Publishing research using ab76237? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1 Review or Q&A

    10
    Votes: Highest First

    Womit wurde IP getestet? Erkennt dieser AK auch denaturiertes Protein?

    Vielen Dank fuer Ihren Anruf.

    Das Labortestet den Cytochrom C-Antikoerper ab76237 mit nicht-denaturiertem COS-1-Zelllysat in der IP, da ab76237 nur natives Cytochrom C erkennt.

    Ich hoffe, dies hilft Ihnen weiter. Bitte zoegern Sie...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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