Anti-Cytochrome C antibody [EP1326-80-5] (ab76107)

Overview

  • Product nameAnti-Cytochrome C antibody [EP1326-80-5]
    See all Cytochrome C primary antibodies
  • Description
    Rabbit monoclonal [EP1326-80-5] to Cytochrome C
  • Tested applicationsSuitable for: WB, IP, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues near the N terminus of human Cytochrome C

  • Positive control
    • HeLa, COS and L929 cell lysates; human kidney tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Associated products

Applications

Our Abpromise guarantee covers the use of ab76107 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 12 kDa.
IP 1/50.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/50 - 1/100.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • FunctionElectron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.
      Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
    • Involvement in diseaseDefects in CYCS are the cause of thrombocytopenia type 4 (THC4) [MIM:612004]; also known as autosomal dominant thrombocytopenia type 4. Thrombocytopenia is the presence of relatively few platelets in blood. THC4 is a non-syndromic form of thrombocytopenia. Clinical manifestations of thrombocytopenia are absent or mild. THC4 may be caused by dysregulated platelet formation.
    • Sequence similaritiesBelongs to the cytochrome c family.
    • Post-translational
      modifications
      Binds 1 heme group per subunit.
    • Cellular localizationMitochondrion matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • CYC antibody
      • CYC_HUMAN antibody
      • CYCS antibody
      • Cytochrome c antibody
      • Cytochrome c somatic antibody
      • HCS antibody
      • THC4 antibody
      see all

    Anti-Cytochrome C antibody [EP1326-80-5] images

    • ab76107 at 1/100 dilution staining Cytochrome C in human kidney by Immunohistochemistry using paraffin-embedded tissue.
    • All lanes : Anti-Cytochrome C antibody [EP1326-80-5] (ab76107) at 1/200 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : COS cell lysate
      Lane 3 : L929 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 12 kDa
      Observed band size : 15 kDa (why is the actual band size different from the predicted?)

    References for Anti-Cytochrome C antibody [EP1326-80-5] (ab76107)

    ab76107 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Quercus suber Cell (Secondary Phloem)
    Permeabilization No
    Specification Secondary Phloem
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Nov 10 2015

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Permeabilization Yes - 0.5% Triton X100 in PBS
    Fixative Paraformaldehyde
    Username

    Dr. Kirk McManus

    Verified customer

    Submitted Apr 04 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"