Recombinant Anti-Cytochrome C antibody [EP1326-80-5] (ab76107)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1326-80-5] to Cytochrome C
- Suitable for: WB, IHC-P, mIHC
- Reacts with: Mouse, Human, African green monkey
Related conjugates and formulations
Overview
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Product name
Anti-Cytochrome C antibody [EP1326-80-5]
See all Cytochrome C primary antibodies -
Description
Rabbit monoclonal [EP1326-80-5] to Cytochrome C -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, mIHCmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Mouse, Human, African green monkey
Predicted to work with: Rat -
Immunogen
Synthetic peptide within Human Cytochrome C (N terminal). The exact sequence is proprietary.
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Positive control
- WB: HeLa, COS and L929 cell lysates. IHC-P: human kidney tissue. mIHC: Human parathyroid gland tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1326-80-5 -
Isotype
IgG -
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipases
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76107 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/500. Predicted molecular weight: 12 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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mIHC |
Use at an assay dependent concentration.
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Notes |
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WB
1/500. Predicted molecular weight: 12 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
mIHC
Use at an assay dependent concentration. |
Target
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Function
Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.
Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases. -
Involvement in disease
Defects in CYCS are the cause of thrombocytopenia type 4 (THC4) [MIM:612004]; also known as autosomal dominant thrombocytopenia type 4. Thrombocytopenia is the presence of relatively few platelets in blood. THC4 is a non-syndromic form of thrombocytopenia. Clinical manifestations of thrombocytopenia are absent or mild. THC4 may be caused by dysregulated platelet formation. -
Sequence similarities
Belongs to the cytochrome c family. -
Post-translational
modificationsBinds 1 heme group per subunit. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 54205 Human
- Entrez Gene: 13063 Mouse
- Entrez Gene: 25309 Rat
- Omim: 123970 Human
- SwissProt: P99999 Human
- SwissProt: P62897 Mouse
- SwissProt: P62898 Rat
- Unigene: 437060 Human
see all -
Alternative names
- CYC antibody
- CYC_HUMAN antibody
- CYCS antibody
see all
Images
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Fluorescence multiplex immunohistochemical analysis of the Human parathyroid gland (Formalin/PFA-fixed paraffin-embedded sections).
Panel A: merged staining of anti-CaSR (ab259846, magenta; Opal™690), anti-Cytochrome C (ab247438, green; Opal™520) and anti-FABP4 (ab92501, red; Opal™570) on human parathyroid gland. Panel B: anti-CaSR stained on parathyroid chief cells. Panel C: anti-Cytochrome C stained on parathyroid oxyphil cells. Panel D: anti-FABP4 stained on adipocytes. Opal Polymer HRP Ms + Rb was used as a secondary antibody.
The section was incubated in three rounds of staining: in the order of ab259846 at 1/5000 dilution (0.103 μg/ml), ab247438 at 1/5000 dilution (0.195 μg/ml), and ab92501 at 1/10000 dilution (0.047 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain.
This data was developed using ab247438, the same antibody clone in a different buffer formulation.
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Fluorescence multiplex immunohistochemical analysis of the human parathyroid gland (Formalin/PFA-fixed paraffin-embedded sections).
Panel A: merged staining of anti-Parathyroid Hormone (ab236229, magenta; Opal™690), anti-Cytochrome C (ab247438, green; Opal™520) and anti-FABP4 (ab92501, red; Opal™570) on human parathyroid gland. Panel B: anti-Cytochrome C stained on parathyroid oxyphil cells. Panel C: anti-Parathyroid Hormone stained on parathyroid chief cells. Panel D: anti-FABP4 stained on adipocytes. Opal Polymer HRP Ms + Rb was used as a secondary antibody.
The section was incubated in three rounds of staining: in the order of ab236229 at 1/200 dilution (5.065 μg/ml) for 10 mins, then ab247438 at 1/5000 dilution (0.195 μg/ml) and ab92501 at 1/10000 dilution (0.047 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. DAPI (blue) was used as a nuclear counter stain.
This data was developed using ab247438, the same antibody clone in a different buffer formulation.
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ab76107 at 1/100 dilution staining Cytochrome C in human kidney by Immunohistochemistry using paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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All lanes : Anti-Cytochrome C antibody [EP1326-80-5] (ab76107) at 1/200 dilution
Lane 1 : HeLa cell lysate
Lane 2 : COS cell lysate
Lane 3 : L929 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Lanes 1-2 : Goat anti-rabbit HRP at 1/2000 dilution
Lane 3 : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 12 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab76107 has been referenced in 7 publications.
- Wu Q et al. Epigallocatechin gallate enhances human lens epithelial cell survival after UVB irradiation via the mitochondrial signaling pathway. Mol Med Rep 25:N/A (2022). PubMed: 35039875
- Cheng F et al. Angiotensin-(1-7) ameliorates high glucose-induced vascular endothelial injury through suppressing chloride channel 3. Bioengineered 13:4100-4111 (2022). PubMed: 35098884
- Zhang Z et al. Hepatic HuR modulates lipid homeostasis in response to high-fat diet. Nat Commun 11:3067 (2020). PubMed: 32546794
- Keshri GK et al. Effects of Pulsed 810?nm Al-Ga-As Diode Laser on Wound Healing Under Immunosuppression: A Molecular Insight. Lasers Surg Med 52:424-436 (2020). PubMed: 31483061
- Jeon SJ et al. TIPRL potentiates survival of lung cancer by inducing autophagy through the eIF2a-ATF4 pathway. Cell Death Dis 10:959 (2019). PubMed: 31862913
- Liu C et al. 16-Hydroxycleroda-3, 13-dien-15, 16-olide inhibits the proliferation and induces mitochondrial-dependent apoptosis through Akt, mTOR, and MEK-ERK pathways in human renal carcinoma cells. Phytomedicine 36:95-107 (2017). PubMed: 29157834
- Xu Z et al. The protective effect of propofol against TNF-a-induced apoptosis was mediated via inhibiting iNOS/NO production and maintaining intracellular Ca2+homeostasis in mouse hippocampal HT22 cells. Biomed Pharmacother 91:664-672 (2017). PubMed: 28499237