Anti-Cytochrome C antibody [SJL2-4(apo)] (ab16076)

Overview

  • Product nameAnti-Cytochrome C antibody [SJL2-4(apo)]
    See all Cytochrome C primary antibodies
  • Description
    Mouse monoclonal [SJL2-4(apo)] to Cytochrome C
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Goat, Human, Monkey
  • Immunogen

    Recombinant full length protein (Horse).

  • Positive control
    • 293T cells

Applications

Our Abpromise guarantee covers the use of ab16076 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 12 kDa.

Target

  • FunctionElectron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.
    Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
  • Involvement in diseaseDefects in CYCS are the cause of thrombocytopenia type 4 (THC4) [MIM:612004]; also known as autosomal dominant thrombocytopenia type 4. Thrombocytopenia is the presence of relatively few platelets in blood. THC4 is a non-syndromic form of thrombocytopenia. Clinical manifestations of thrombocytopenia are absent or mild. THC4 may be caused by dysregulated platelet formation.
  • Sequence similaritiesBelongs to the cytochrome c family.
  • Post-translational
    modifications
    Binds 1 heme group per subunit.
  • Cellular localizationMitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • CYC antibody
    • CYC_HUMAN antibody
    • CYCS antibody
    • Cytochrome c antibody
    • Cytochrome c somatic antibody
    • HCS antibody
    • THC4 antibody
    see all

Anti-Cytochrome C antibody [SJL2-4(apo)] images

  • Western blot of human Cytochrome C from 293T cells (divided into two fractions: Lane 1- cytoplasmic, Lane 2-nuclear) using anti Cytochrome c antibody (ab16076) at 1µg/mL.

    Method: Cell extracts from 293T cells (2x106) were separated into cytoplasmic and nuclear fraction, resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the monoclonal anti-cytochrome c antibody (ab16076) at 1µg/ml. Proteins were visualized using a peroxidase-conjugated antibody to mouse IgG and a chemiluminescence detection system.

References for Anti-Cytochrome C antibody [SJL2-4(apo)] (ab16076)

This product has been referenced in:
  • Corradin G  et al. Differential effects of two anti-apo-cytochrome c-specific monoclonal antibodies on the function of apo-cytochrome c-specific murine T cell clones. J Immunol 133:2915-9 (1984). Read more (PubMed: 6208267) »

See 1 Publication for this product

Product Wall

Thank you for your enquiry. This antibody should work well in IP applications. As decribed in details in the literature specific reference of this product (Differential effects of two anti-apo-cytochrome c-specific monoclonal antibodies on the function...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"