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Anti-Cytochrome C oxidase subunit II antibody [12C4F12 ] (ab110258)

MSCatalog No. MS405

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Overview

Product name

Anti-Cytochrome C oxidase subunit II antibody [12C4F12 ]
See all Cytochrome C oxidase subunit II products (6) ...

Description

Mouse monoclonal [12C4F12 ] to Cytochrome C oxidase subunit II

Tested applications

IHC-P, WB, ICC/IF, Flow Cytmore details

Cross reactivity

Reacts with

Human

Immunogen

Full length native Human Cytochrome C oxidase subunit II purified by SDS PAGE.

Positive control

Isolated mitochondria from Human heart; Cultured Human embryonic lung derived fibroblasts; HeLa cells.

Properties

Form

Liquid

Storage instructions

Store at +4°C.

Storage buffer

Preservative: 0.02% Sodium azide
Constituent: HBS

Concentration

Concentration information loading...

Purity

IgG fraction

Purification notes

ab110258 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. ab110258 was judged near homogeneity by SDS-PAGE.

Clonality

Monoclonal

Clone number

12C4F12

Isotype

IgG2a

Light chain type

kappa

Research areas

Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Cytochromes
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Oxidative phosphorylation >> Complex IV
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Energy transfer pathways >> Integration of energy
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Integration of energy metabolism
Signal Transduction >> Metabolism >> Mitochondrial

  • Western blot - Cytochrome C oxidase subunit II antibody [12C4F12 ] (ab110258)Western blot - Cytochrome C oxidase subunit II antibody [12C4F12 ] (ab110258) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - Cytochrome C oxidase subunit II antibody [12C4F12 ] (ab110258)Immunocytochemistry/ Immunofluorescence - Cytochrome C oxidase subunit II antibody [12C4F12 ] (ab110258) image (enlarge)

  • Flow Cytometry - Cytochrome C oxidase subunit II antibody [12C4F12 ] (ab110258)Flow Cytometry - Cytochrome C oxidase subunit II antibody [12C4F12 ] (ab110258) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab110258 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1.

Involvement in disease

Defects in MT-CO2 are a cause of mitochondrial complex IV deficiency (MT-C4D) [MIM:220110]; also known as cytochrome c oxidase deficiency. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations, ranging from isolated myopathy to severe multisystem disease affecting several tissues and organs. Features include hypertrophic cardiomyopathy, hepatomegaly and liver dysfunction, hypotonia, muscle weakness, excercise intolerance, developmental delay, delayed motor development and mental retardation. A subset of patients manifest Leigh syndrome.

Sequence similarities

Belongs to the cytochrome c oxidase subunit 2 family.

Cellular localization

Mitochondrion inner membrane.

Target information above from: UniProt accessionP00403 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • COII antibody
  • COX2 antibody
  • COX2_HUMAN antibody
  • COXII antibody
  • Cytochrome c oxidase II antibody
  • Cytochrome c oxidase polypeptide II antibody
  • Cytochrome c oxidase subunit 2 antibody
  • MS405 antibody
  • MS419 antibody
  • MT CO2 antibody
  • MT-CO2 antibody
  • MTCO2 antibody
see all

Database links

Anti-Cytochrome C oxidase subunit II antibody [12C4F12 ] images:

  Western blot - Cytochrome C oxidase subunit II antibody [12C4F12 ] (ab110258)

Western blot - Cytochrome C oxidase subunit II antibody [12C4F12 ] (ab110258)

Anti-Cytochrome C oxidase subunit II antibody [12C4F12 ] (ab110258) at 1 µg/ml + Human Heart Mitochondrial lysate (ab110337) at 5 µg

Predicted band size : 26 kDa

  Immunocytochemistry/ Immunofluorescence - Cytochrome C oxidase subunit II antibody [12C4F12 ] (ab110258)

Immunocytochemistry/ Immunofluorescence - Cytochrome C oxidase subunit II antibody [12C4F12 ] (ab110258)

ab110258, at 5 µg/ml, staining Cytochrome C oxidase subunit II in Human embryonic lung derived fibroblasts (MRC5) by Immunofluorescence. An AlexaFluor® 488 conjugated Goat anti-mouse IgG2a isotype specific secondary antibody was used at 2 µg/ml.

  Flow Cytometry - Cytochrome C oxidase subunit II antibody [12C4F12 ] (ab110258)

Flow Cytometry - Cytochrome C oxidase subunit II antibody [12C4F12 ] (ab110258)

ab110258, at 1 µg/ml, staining Cytochrome C oxidase subunit II in HeLa cells by Flow Cytometry (Blue).

An isotype control antibody, at 1 µg/ml, staining in HeLa cells (Red).

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C oxidase subunit II antibody [12C4F12 ] (ab110258)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C oxidase subunit II antibody [12C4F12 ] (ab110258)

ab110258 staining human lung sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab110258 at 1/250 in TBS/BSA/azide for 2h at 21°C. A biotinylated goat anti-mouse polyclonal antibody at 1/200 was used as the secondary antibody.

This image is courtesy of an Abreview submitted by Carl Hobbs

See Abreview

References for Anti-Cytochrome C oxidase subunit II antibody [12C4F12 ] (ab110258)

This product has been referenced in:

  • Leadsham JE & Gourlay CW cAMP/PKA signaling balances respiratory activity with mitochondria dependent apoptosis via transcriptional regulation. BMC Cell Biol 11:92 (2010).Read more (PubMed: 21108829) »
  • Gegg ME  et al. Mitofusin 1 and mitofusin 2 are ubiquitinated in a PINK1/parkin-dependent manner upon induction of mitophagy. Hum Mol Genet 19:4861-70 (2010).Read more (PubMed: 20871098) »

See all 58 publications for this product

Publishing research using ab110258? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"