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Tanzania, United Republic of
Antigua and Barbuda
Saint Kitts and Nevis
Saint Pierre and Miquelon
Trinidad & Tob
Korea, Rep of
Papua New Guinea
Bosnia and Herzegovina
ab110172 (MSA41) is a 96-well microplate assay that is used to determine the amount of cytochrome c in a human, mouse, rat or bovine sample. Cytochrome c is immunocaptured within the wells and the amount is determined by adding a cytochrome c specific antibody conjugated with horseradish peroxidase. This peroxidase changes the substrate from colorless to blue at 600 nm. This reaction takes place in a time dependent manner proportional to the amount of protein captured in the wells. The rate of development of blue color can be followed at 600 nm or the reaction can be stopped at a user defined time by the addition of 100 µl of 1.5N HCl to each well (not supplied) and read at 450 nm. Included for the performance of this quantity assay are buffers, detergent, substrates, and 96-well microplate with monoclonal antibody pre-bound to the wells of the plate, allowing for a stream-lined assay.
|10X Blocking Buffer||1 x 15ml|
|20X Buffer||Tube 1||1 x 15ml|
|20X Detector antibody||Tube A||1 x 1ml|
|20X HRP label||Tube B||1 x 1ml|
|20X Wash Buffer||Tube 3||1 x 15ml|
|96-well pre-coated microplate||1 unit|
|Detergent||1 x 1ml|
|HRP Development Solution||1 x 20ml|
Our Abpromise guarantee covers the use of ab110172 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent dilution.|
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"