Overview

  • Product nameCytochrome c Quantitative ELISASee all Cytochrome c kits ...
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Mouse liver 3 = 5.6%
    Inter-assay
    Sample n Mean SD CV%
    Mouse liver 2 = 0.3%
  • Sample type
    Tissue Extracts, Cell Lysate
  • Assay typeSandwich (quantitative)
  • Sensitivity
    = 7.22 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture extracts 4µg/ml - 100µg/ml
    Tissue Homogenate 4µg/ml - 100µg/ml
    Purified protein 6ng/ml - 200ng/ml

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Product overview

    ab154471 is an in vitro enzyme-linked immunosorbent assay (ELISA) for the comparison of cytochrome C levels or profile in cell or tissue lysates from different species.

    The assay employs a Cytochrome c specific antibody coated onto microplate strips. Standards or samples are pipetted into the wells and Cytochrome c present in the sample is bound to the wells by the immobilized antibody. The wells are washed and an anti-Cytochrome c detector antibody is added. After washing away unbound detector antibody, a HRP conjugated antibody is pipetted into the wells. The wells are again washed, an HRP substrate solution (TMB) is added to the wells and color develops in proportion to the amount of Cytochrome c bound. The developing blue color is measured at 600 nm. Optionally the reaction can be stopped by adding hydrochloric acid which changes the color from blue to yellow and the intensity can be measured at 450 nm.

  • Notes

    Cytochrome c is a water-soluble protein of 12 kDa, residing loosely attached in the mitochondrial intermembrane space. As an electron carrier protein, Cytochrome c functions in the respiratory chain by interaction with redox partners. Cytochrome c also participates in apoptosis. The release of cytochrome c into the cytosol leads to an activation of an apoptotic program via activation of a caspase dependent pathway. Cytochrome c achieves this goal by interaction with other cytosolic factors forming a complex (apoptosome) composed of cytochrome c, Apaf-1, dATP and Apaf-3/caspase 9. Bcl-2 on the other hand was shown to be able to prevent apoptosis by blocking the release of Cytochrome c from mitochondria. Measurement of Cytochrome c release from the mitochondria is a tool to detect the first early steps for initiating apoptosis in cells. Moreover, elevated Cytochrome c levels were observed in serum from patients with hematological malignancies.

  • Tested applicationsELISAmore details
  • PlatformMicroplate

Properties

  • RelevanceElectron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
  • Cellular localizationMitochondrion matrix
  • Alternative names
    • CYC
    • CYCS
    • Cytochrome c somatic
    • HCS
    • THC4
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab154471 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Cytochrome c Quantitative ELISA images

  • A dilution series of Human HepG2 cells extract (A), Rat Heart Homogenate extract (B), and Mouse Liver Homogenate extract (C).
  • A dilution series of Cytochrome c protein in Incubation Buffer in the working range of the assay

Protocols

References for Cytochrome c Quantitative ELISA (ab154471)

This product has been referenced in:
  • Salman ZK  et al. The combined effect of metformin and L-cysteine on inflammation, oxidative stress and insulin resistance in streptozotocin-induced type 2 diabetes in rats. Eur J Pharmacol 714:448-55 (2013). Read more (PubMed: 23845213) »
  • Hollie NI  et al. Micromolar changes in lysophosphatidylcholine concentration cause minor effects on mitochondrial permeability but major alterations in function. Biochim Biophys Acta 1841:888-895 (2013). Mouse . Read more (PubMed: 24315825) »

See all 2 Publications for this product

Product Wall

The detergent in the extraction buffer is an ionic surfactant which is capable to lyse all kinds of cellular membranes and extract most of the proteins in a cell. The specific components of the buffer are proprietary.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"