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|Mouse liver||3||= 5.6%|
|Sample type||Average %||Range|
|Cell culture extracts||4µg/ml - 100µg/ml|
|Tissue Homogenate||4µg/ml - 100µg/ml|
|Purified protein||6ng/ml - 200ng/ml|
ab154471 is an in vitro enzyme-linked immunosorbent assay (ELISA) for the comparison of cytochrome C levels or profile in cell or tissue lysates from different species.
The assay employs a Cytochrome c specific antibody coated onto microplate strips. Standards or samples are pipetted into the wells and Cytochrome c present in the sample is bound to the wells by the immobilized antibody. The wells are washed and an anti-Cytochrome c detector antibody is added. After washing away unbound detector antibody, a HRP conjugated antibody is pipetted into the wells. The wells are again washed, an HRP substrate solution (TMB) is added to the wells and color develops in proportion to the amount of Cytochrome c bound. The developing blue color is measured at 600 nm. Optionally the reaction can be stopped by adding hydrochloric acid which changes the color from blue to yellow and the intensity can be measured at 450 nm.
Cytochrome c is a water-soluble protein of 12 kDa, residing loosely attached in the mitochondrial intermembrane space. As an electron carrier protein, Cytochrome c functions in the respiratory chain by interaction with redox partners. Cytochrome c also participates in apoptosis. The release of cytochrome c into the cytosol leads to an activation of an apoptotic program via activation of a caspase dependent pathway. Cytochrome c achieves this goal by interaction with other cytosolic factors forming a complex (apoptosome) composed of cytochrome c, Apaf-1, dATP and Apaf-3/caspase 9. Bcl-2 on the other hand was shown to be able to prevent apoptosis by blocking the release of Cytochrome c from mitochondria. Measurement of Cytochrome c release from the mitochondria is a tool to detect the first early steps for initiating apoptosis in cells. Moreover, elevated Cytochrome c levels were observed in serum from patients with hematological malignancies.
|Components||1 x 96 tests|
|10X Blocking Buffer||1 x 6ml|
|10X Cytochrome C Detector Antibody||1 x 1ml|
|10X Dilution Buffer||1 x 1.5ml|
|10X Extraction Buffer||1 x 1.5ml|
|10X HRP Label||1 x 1ml|
|20X Buffer||1 x 20ml|
|Cytochrome C Microplate||1 x 96 units|
|Cytochrome C standard protein||1 x 100µg|
|TMB Development Solution||1 x 12ml|
Our Abpromise guarantee covers the use of ab154471 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"