The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty.
Lipid metabolism; steroid biosynthesis.
Involvement in disease
Defects in CYP17A1 are the cause of adrenal hyperplasia type 5 (AH5) [MIM:202110]. AH5 is a form of congenital adrenal hyperplasia, a common recessive disease due to defective synthesis of cortisol. Congenital adrenal hyperplasia is characterized by androgen excess leading to ambiguous genitalia in affected females, rapid somatic growth during childhood in both sexes with premature closure of the epiphyses and short adult stature. Four clinical types: "salt wasting" (SW, the most severe type), "simple virilizing" (SV, less severely affected patients), with normal aldosterone biosynthesis, "non-classic form" or late onset (NC or LOAH), and "cryptic" (asymptomatic).
Belongs to the cytochrome P450 family.
Phosphorylation is necessary for 17,20-lyase, but not for 17-alpha-hydroxylase activity.
Western blot - Cytochrome P450 17A1 antibody (ab64886)
All lanes : Anti-Cytochrome P450 17A1 antibody (ab64886) at 1/500 dilution
Lane 1 : extracts from A549 cells (5-30µg total protein) Lane 2 : extracts from Jurkat cells (5-30µg total protein) Lane 3 : extracts from HeLa cells (5-30µg total protein) Lane 4 : extracts from HeLa cells (5-30µg total protein) and 5-10µg of the immunising peptide.
Predicted band size : 57 kDa Observed band size : 57 kDa Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.