Anti-Cytochrome P450 17A1 antibody [EPR6293] (ab125022)


  • Product name
    Anti-Cytochrome P450 17A1 antibody [EPR6293]
    See all Cytochrome P450 17A1 primary antibodies
  • Description
    Rabbit monoclonal [EPR6293] to Cytochrome P450 17A1
  • Tested applications
    Suitable for: WB, IP, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Cytochrome P450 17A1 aa 100-200. The exact sequence is proprietary.
    Database link: P05093

  • Positive control
    • Human adrenal gland, SK-OV-3, HeLa and Jurkat lysates; HeLa cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.


Our Abpromise guarantee covers the use of ab125022 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 57 kDa).
IP 1/10 - 1/100.
Flow Cyt Use at an assay dependent concentration.

ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty.
    • Pathway
      Lipid metabolism; steroid biosynthesis.
    • Involvement in disease
      Defects in CYP17A1 are the cause of adrenal hyperplasia type 5 (AH5) [MIM:202110]. AH5 is a form of congenital adrenal hyperplasia, a common recessive disease due to defective synthesis of cortisol. Congenital adrenal hyperplasia is characterized by androgen excess leading to ambiguous genitalia in affected females, rapid somatic growth during childhood in both sexes with premature closure of the epiphyses and short adult stature. Four clinical types: "salt wasting" (SW, the most severe type), "simple virilizing" (SV, less severely affected patients), with normal aldosterone biosynthesis, "non-classic form" or late onset (NC or LOAH), and "cryptic" (asymptomatic).
    • Sequence similarities
      Belongs to the cytochrome P450 family.
    • Post-translational
      Phosphorylation is necessary for 17,20-lyase, but not for 17-alpha-hydroxylase activity.
    • Cellular localization
    • Information by UniProt
    • Database links
    • Alternative names
      • 20 lyase antibody
      • CP17A_HUMAN antibody
      • CPT7 antibody
      • CYP17 antibody
      • CYP17A1 antibody
      • CYPXVII antibody
      • Cytochrome P450 17A1 antibody
      • Cytochrome P450 family 17 antibody
      • Cytochrome P450 family 17 subfamily A polypeptide 1 antibody
      • Cytochrome p450 subfamily XVII (steroid 17 alpha hydroxylase) adrenal hyperplasia antibody
      • Cytochrome p450 XVIIA1 antibody
      • Cytochrome P450-C17 antibody
      • Cytochrome P450c17 antibody
      • OTTHUMP00000020382 antibody
      • P450 C17 antibody
      • P450c17 antibody
      • S17AH antibody
      • Steroid 17 alpha hydroxylase/17,20 lyase antibody
      • Steroid 17 alpha monooxygenase antibody
      • Steroid 17-alpha-hydroxylase/17 antibody
      • Steroid 17-alpha-monooxygenase antibody
      see all


    • ab125022, at 1/100 dilution, staining Cytochrome P450 17A1 in Hela cells by Immunofluorescence.
    • All lanes : Anti-Cytochrome P450 17A1 antibody [EPR6293] (ab125022) at 1/1000 dilution

      Lane 1 : Human adrenal gland lysate
      Lane 2 : SK-OV-3 lysate
      Lane 3 : HeLa lysate
      Lane 4 : Jurkat lysate

      Lysates/proteins at 10 µg per lane.

      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 57 kDa
      Observed band size : 55 kDa (why is the actual band size different from the predicted?)
    • Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Cytochrome P450 17A1 with purified ab125022 at 1/220 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.


    This product has been referenced in:
    • Wu S  et al. Obesity-induced infertility and hyperandrogenism are corrected by deletion of the insulin receptor in the ovarian theca cell. Diabetes 63:1270-82 (2014). Mouse . Read more (PubMed: 24379345) »
    • Dowling AR  et al. Genetic factors modulate the impact of pubertal androgen excess on insulin sensitivity and fertility. PLoS One 8:e79849 (2013). WB ; Mouse . Read more (PubMed: 24278193) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    Western blot
    Human Cell lysate - whole cell (Prostate Cancer Cell Lines)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    30 µg
    Hormone treatment
    Prostate Cancer Cell Lines
    Blocking step
    Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C

    Ms. Virginia Fasoulaki

    Verified customer

    Submitted Sep 27 2017

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Immunocytochemistry/ Immunofluorescence
    Human Cell (H1299 Lung cancer cell line)
    Yes - 0.1% v/v TritonX-100
    H1299 Lung cancer cell line
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C

    Dr. Dimitra Kalamida

    Verified customer

    Submitted May 31 2016


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