Anti-Cytochrome P450 1A1 + 1A2 antibody (ab4227)


  • Product nameAnti-Cytochrome P450 1A1 + 1A2 antibody
    See all Cytochrome P450 1A1 + 1A2 primary antibodies
  • Description
    Goat polyclonal to Cytochrome P450 1A1 + 1A2
  • SpecificityReacts with both forms in liver microsomes prepared from rabbits or rats that were induced to express multiple forms of P450’s.
  • Tested applicationsSuitable for: WB, ELISA, IPmore details
  • Species reactivity
    Reacts with: Rat, Rabbit
  • Immunogen

    Full length native CYP1A1 and CYP1A2 (purified) (Rabbit).

  • Positive control
    • WB: 0.2 µg of recombinant human cytochrome P450.
  • General notes

    The Cytochrome P450 (P450) superfamily of enzymes is one of three enzyme systems which metabolize the fatty acid arachadonic acid (AA) to regulators of vascular tone. P450 enzymes are monooxygenase enzymes which require several co-factors such as nicotinamide adenine dinucleotide phosphate (NADPH) and P450 reductase. There are over 200 known genes which encode P450s. Epoxygenases are those P450s which metabolize AA to epoxyeicosatrienoic acid (EETs) and omega-hydroxylases are those P450s which produce 19- and 20-hydroxyeicosatetraenoic acids (19- and 20-HETE). As well as fatty acid metabolism, P450s also metabolize many drugs and toxins. Cytochrome P450 3A4 is abundantly expressed in liver and small intestine and is inducible by barbiturates, glucocorticoids and rifampicin.



Our Abpromise guarantee covers the use of ab4227 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100. Predicted molecular weight: 54,56 kDa.

Predicted molecular weight of 1A1: 54 kDa; predicted molecular weight of 1A2: 56 kDa.

ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration.


  • RelevanceCytochrome P450 oxidase (commonly abbreviated CYP) is a generic term for a large number of related, but distinct, oxidative enzymes important in vertebrate physiology. The cytochrome P450 mixed-function monooxygenase system is probably the most important element of Phase I metabolism in mammals. P450s are membrane-bound, either in the inner membrane of mitochondria or in the endoplasmic reticulum of cells where they metabolise thousands of endogenous and exogenous compounds. In the liver, these substrates include toxins, drugs, and other unneeded and potentially harmful molecules. Humans have 18 families of cytochrome P450 genes and 43 subfamilies; the CYP1 family is involved in drug metabolism and includes 3 subfamilies, 3 genes and 1 pseudogene.
  • Cellular localizationEndoplasmic reticulum
  • Database links
  • Alternative names
    • Aryl hydrocarbon hydroxylase antibody
    • CP11 antibody
    • CP12 antibody
    • CYP1A1 antibody
    • CYP1A2 antibody
    • CYPIA1 antibody
    • CYPIA2 antibody
    • Cytochrome P450 family 1 subfamily A polypeptide 1 antibody
    • Cytochrome P450 family 1 subfamily A polypeptide 2 antibody
    • Cytochrome P450 subfamily I aromatic compound inducible polypeptide 1 antibody
    • Cytochrome P450 subfamily I aromatic compound inducible polypeptide 2 antibody
    • P1 450 antibody
    • P3 450 antibody
    • P450 form 4 antibody
    • P450 form 6 antibody
    • P450 P1 antibody
    • P450 P3 antibody
    see all

Anti-Cytochrome P450 1A1 + 1A2 antibody images

  • ab4227 used in Immunoprecipitation.
    The systems were cross-linked (X-L) with BS3, immunoprecipitated (IP) with ab4227, subjected to SDS-PAGE, and blotted with anti-CYP1A2.

    Each of the reconstituted systems was cross-linked with the water-soluble, homobifunctional cross-linking agent BS3, which reacts with primary amino groups. Each 100µl reaction mixture was treated with 1 mM BS3 for 2 minutes at 4 °C and then quenched with 96µl of ice-cold 2radioimmune precipitation buffer and 500 mM glycine and placed in an ice bath. Samples remained on ice for 15 minutes before the addition of 4µl of ab4227. Samples were then allowed to incubate for 2 hours on ice. At the end of the 2-hour incubation, 100µl of 25% (w/v) Protein G-agarose bead slurry was added, and each sample was rocked/incubated for 1 hour at 4 °C. Samples were spun for 10 seconds at 3500g, the supernatants were transferred to clean microcentrifuge (1.5-ml) tubes, and the bead pellets were washed four times with 100µl of radioimmune precipitation buffer. After aspirating the final wash, 100µl of loading buffer was added to each sample. The samples were treated with 5µl of beta-mercaptoethanol before boiling for 10 minutes. 30µl of each sample was loaded onto 4–12% (w/v) SDS-polyacrylamide gels and run for 45 minutes at 30mA before running an additional 50 minutes at 200 V.The gels were transferred to nitrocellulose membranes and subjected to immune blot analysis.

References for Anti-Cytochrome P450 1A1 + 1A2 antibody (ab4227)

This product has been referenced in:
  • Brignac-Huber L  et al. Organization of NADPH-Cytochrome P450 Reductase and CYP1A2 in the Endoplasmic Reticulum--Microdomain Localization Affects Monooxygenase Function. Mol Pharmacol 79:549-57 (2011). WB ; Rabbit . Read more (PubMed: 21156755) »
  • Reed JR  et al. Functional interactions between cytochromes P450 1A2 and 2B4 require both enzymes to reside in the same phospholipid vesicle: evidence for physical complex formation. J Biol Chem 285:8942-52 (2010). IP . Read more (PubMed: 20071338) »

See all 2 Publications for this product

Product Wall

Thank you for your enquiry. If you load 0.2 µg of recombinant human cytochrome P450 (positive control), we would suggest using 1/100 dilution. The dilution depends on the type of the samples, the expressed levels of the target protein, detection sys...

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