The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: 1/300 - 1/1000.
IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol (see references).
WB: 1/50 - 1/500. Predicted molecular weight: 55 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceCytochrome P450 oxidase (commonly abbreviated CYP) is a generic term for a large number of related, but distinct, oxidative enzymes important in vertebrate physiology. The cytochrome P450 mixed-function monooxygenase system is probably the most important element of Phase I metabolism in mammals. P450s are membrane-bound, either in the inner membrane of mitochondria or in the endoplasmic reticulum of cells where they metabolise thousands of endogenous and exogenous compounds. In the liver, these substrates include toxins, drugs, and other unneeded and potentially harmful molecules. Humans have 18 families of cytochrome P450 genes and 43 subfamilies; the CYP1 family is involved in drug metabolism and includes 3 subfamilies, 3 genes and 1 pseudogene.
Cellular localizationEndoplasmic reticulum
Aryl hydrocarbon hydroxylase antibody
Cytochrome P450 family 1 subfamily A polypeptide 1 antibody
Cytochrome P450 family 1 subfamily A polypeptide 2 antibody
Cytochrome P450 subfamily I aromatic compound inducible polypeptide 1 antibody
Cytochrome P450 subfamily I aromatic compound inducible polypeptide 2 antibody