Overview

  • Product nameAnti-Cytochrome P450 1A1 antibody
    See all Cytochrome P450 1A1 primary antibodies
  • Description
    Rabbit polyclonal to Cytochrome P450 1A1
  • SpecificityDetects Cytochrome P450 1A1 from Human cells. This antibody detects, to a lesser extent, other P450 isoforms.
  • Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Other Immunogen Type (His-tag) corresponding to Human Cytochrome P450 1A1. Purified, His-tagged, full length human P450 1A1 fusion protein.

  • Positive control
    • MCF-7 cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab3568 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/1000.
WB 1/500.

By Western blot, this antibody detects an ~54 kDa protein representing P450 1A1 from MCF-7 cell extract.

Target

  • FunctionCytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
  • Tissue specificityLung, lymphocytes and placenta.
  • Sequence similaritiesBelongs to the cytochrome P450 family.
  • Cellular localizationEndoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AHH antibody
    • AHRR antibody
    • Aryl hydrocarbon hydroxylase antibody
    • CP11 antibody
    • CP1A1_HUMAN antibody
    • CYP 1 antibody
    • CYP1 antibody
    • cyp1a1 antibody
    • CYPIA1 antibody
    • Cytochrome P1 450 dioxin inducible antibody
    • Cytochrome P1-450 antibody
    • Cytochrome P450 1A1 antibody
    • Cytochrome P450 family 1 subfamily A polypeptide 1 antibody
    • Cytochrome P450 form 6 antibody
    • Cytochrome P450 subfamily I (aromatic compound inducible) polypeptide 1 antibody
    • Cytochrome P450-C antibody
    • Cytochrome P450-P1 antibody
    • Flavoprotein-linked monooxygenase antibody
    • Microsomal monooxygenase antibody
    • P1 450 antibody
    • P450 C antibody
    • P450 form 6 antibody
    • P450 P1 antibody
    • P450DX antibody
    • Xenobiotic monooxygenase antibody
    see all

Anti-Cytochrome P450 1A1 antibody images

  • ab3568 at a dilution of 1/500 staining MCF-7 cell extract by Western blot.

  • ICC/IF image of ab3568 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3568, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab3568 staining in Human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3568, 1/200 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • HepG2 cells were incubated at 37°C for 24h with vehicle control (0 µM) and different concentrations of itraconazole (ab120816). Increased expression of cytochrome P450 1A1 (ab3568) in HepG2 cells correlates with an increase in nifuroxazide concentration, as described in literature.

    Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 3% milk before being incubated with ab3568 at 1/500 dilution and ab8227 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.


  • Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 16 minutes

    HepG2 cells were incubated at 37°C for 24h with vehicle control (0 µM) and 100 µM of fluconazole (ab141065). Increased expression of cytochrome P450 1A1 (ab3568) correlates with an increase in fluconazole concentration, as described in literature.

    Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 3% milk before being incubated with ab3568 at 1/500 dilution and ab8227 at 1 µg /ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.

References for Anti-Cytochrome P450 1A1 antibody (ab3568)

This product has been referenced in:
  • Pramanik D  et al. Restitution of tumor suppressor microRNAs using a systemic nanovector inhibits pancreatic cancer growth in mice. Mol Cancer Ther 10:1470-80 (2011). WB ; Human . Read more (PubMed: 21622730) »
  • Tan BS  et al. CYP2S1 and CYP2W1 mediate 2-(3,4-dimethoxyphenyl)-5-fluorobenzothiazole (GW-610, NSC 721648) sensitivity in breast and colorectal cancer cells. Mol Cancer Ther 10:1982-92 (2011). WB ; Human . Read more (PubMed: 21831963) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us. The black dots on a blot usually indicate that the antibody is binding to the blocking reagent and it needs to be filtered. The 5% milk should be in TBST. I know you have said that you have used your protocol sucessfully wi...

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Thank you for the Western blot images that you have provided. Ab3568 was characterized for application in Western blotting using MCF-7 cell lysate and a band at approximately 54 kDa was detected. You are having a problem with non-specific bands even wi...

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Thank you for your enquiry. The concentration of ab3568 (Cytochrome P450 1A1) is ~3.1 mg/ml. Please let me know if I can provide further assistance.

Thank you for the details that you have provided and I'm sorry to hear that you are experiencing difficulty with ab3568. You mentioned that you saw positive staining with in MCF-7 cells and with commercial CYP1A1 microsomes; with these samples did you ...

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Thank you for your enquiry. Well done on getting your western blot to work. I have read your comments and have examined your blot images. I would like ot take each of your antibodies one at a time: ab3568 - Cytochrome P450 1A1 antibody (ab3...

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