Overview

  • Product nameAnti-Cytochrome P450 1A1 antibody
    See all Cytochrome P450 1A1 primary antibodies
  • Description
    Rabbit polyclonal to Cytochrome P450 1A1
  • Tested applicationsSuitable for: IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide from the C terminal region of Human Cytochrome P450 1A1, conjugated to KLH

  • Positive control
    • Lysates from K562 cell line or Mouse lung tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab79819 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/50.
WB 1/50 - 1/100. Predicted molecular weight: 58 kDa.
ELISA 1/1000.

Target

  • FunctionCytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
  • Tissue specificityLung, lymphocytes and placenta.
  • Sequence similaritiesBelongs to the cytochrome P450 family.
  • Cellular localizationEndoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AHH antibody
    • AHRR antibody
    • Aryl hydrocarbon hydroxylase antibody
    • CP11 antibody
    • CP1A1_HUMAN antibody
    • CYP 1 antibody
    • CYP1 antibody
    • cyp1a1 antibody
    • CYPIA1 antibody
    • Cytochrome P1 450 dioxin inducible antibody
    • Cytochrome P1-450 antibody
    • Cytochrome P450 1A1 antibody
    • Cytochrome P450 family 1 subfamily A polypeptide 1 antibody
    • Cytochrome P450 form 6 antibody
    • Cytochrome P450 subfamily I (aromatic compound inducible) polypeptide 1 antibody
    • Cytochrome P450-C antibody
    • Cytochrome P450-P1 antibody
    • Flavoprotein-linked monooxygenase antibody
    • Microsomal monooxygenase antibody
    • P1 450 antibody
    • P450 C antibody
    • P450 form 6 antibody
    • P450 P1 antibody
    • P450DX antibody
    • Xenobiotic monooxygenase antibody
    see all

Anti-Cytochrome P450 1A1 antibody images

  • Anti-Cytochrome P450 1A1 antibody (ab79819) at 1/1000 dilution + K562 whole cell lysate at 35 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size : 58 kDa

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling P450 1A1 - C-terminal with ab79819 at 1/25. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at 37°C. Heat mediated antigen retrieval was performed using a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hour at 37°C. A Peroxidase-conjugated Goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.

  • Anti-Cytochrome P450 1A1 antibody (ab79819) at 1/50 dilution + Mouse lung tissue lysate at 35 µg

    Predicted band size : 58 kDa

References for Anti-Cytochrome P450 1A1 antibody (ab79819)

This product has been referenced in:
  • Navarro-Tableros V  et al. Recellularization of rat liver scaffolds by human liver stem cells. Tissue Eng Part A 21:1929-39 (2015). IHC-Fr ; Human . Read more (PubMed: 25794768) »
  • Jacob A  et al. Aryl hydrocarbon receptor-dependent upregulation of Cyp1b1 by TCDD and diesel exhaust particles in rat brain microvessels. Fluids Barriers CNS 8:23 (2011). WB ; Rat . Read more (PubMed: 21867498) »

See all 2 Publications for this product

Product Wall

Application Western blot
Sample Rat Tissue lysate - other (brain capillary membranes)
Loading amount 5 µg
Specification brain capillary membranes
Treatment Roche Diagnostics Complete Protease Inhibitor
Gel Running Conditions Reduced Denaturing (Invitrogen NuPage Bis-Tris 4-12%, 1 mm x 15 wells)
Blocking step Pierce Protein-free T20 (TBS) Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
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Submitted Mar 02 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"