Overview

  • Product name
    Anti-Cytochrome P450 1A2 antibody [3B8C1]
    See all Cytochrome P450 1A2 primary antibodies
  • Description
    Mouse monoclonal [3B8C1] to Cytochrome P450 1A2
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Rat Cytochrome P450 1A2 proteins

  • Positive control
    • IHC-P: Normal human liver sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab56073 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
IHC-P Use a concentration of 1 - 5 µg/ml.

Target

  • Function
    Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation. Caffeine is metabolized primarily by cytochrome CYP1A2 in the liver through an initial N3-demethylation. Also acts in the metabolism of aflatoxin B1 and acetaminophen. Participates in the bioactivation of carcinogenic aromatic and heterocyclic amines. Catalizes the N-hydroxylation of heterocyclic amines and the O-deethylation of phenacetin.
  • Tissue specificity
    Liver.
  • Sequence similarities
    Belongs to the cytochrome P450 family.
  • Cellular localization
    Endoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Aryl hydrocarbon hydroxylase antibody
    • CP 12 antibody
    • CP12 antibody
    • CP1A2_HUMAN antibody
    • CYP1A2 antibody
    • CYPIA2 antibody
    • Cytochrome P(3)450 antibody
    • Cytochrome P450 1A2 antibody
    • Cytochrome P450 4 antibody
    • Cytochrome P450 family 1 polypeptide 2 antibody
    • Cytochrome P450 family 1 subfamily A polypeptide 2 antibody
    • Cytochrome P450 subfamily I aromatic compound inducible polypeptide 2 antibody
    • Cytochrome P450-P3 antibody
    • Cytochrome P4501A2 antibody
    • Dioxin inducable P3 450 antibody
    • Flavoprotein linked monooxygenase antibody
    • Microsomal monooxygenase antibody
    • P(3)450 antibody
    • P3 450 antibody
    • P450 4 antibody
    • P450 form 4 antibody
    • P450 P3 antibody
    • P450(PA) antibody
    • Xenobiotic monooxygenase antibody
    see all

Images

  • All lanes : Anti-Cytochrome P450 1A2 antibody [3B8C1] (ab56073)

    Lane 2 : recombinant CYP1B1 at 0.5pmol/lane
    Lane 3 : recombinant CYP1A1 at 0.5pmol/lane
    Lane 4 : recombinant CYP1A2 at 0.5pmol/lane


    Predicted band size : 58 kDa
    Observed band size : 58 kDa
  • Immunohistochemical staining of formalin-fixed, paraffin-embedded normal human liver tissue section using ab56073.

References

This product has been referenced in:
  • Zhang X  et al. A tryptophan derivative, ITE, enhances liver cell metabolic functions in vitro. Int J Mol Med 39:101-112 (2017). Read more (PubMed: 27959388) »
  • Gustafsson F  et al. A correlation between the in vitro drug toxicity of drugs to cell lines that express human P450s and their propensity to cause liver injury in humans. Toxicol Sci 137:189-211 (2014). WB ; Human . Read more (PubMed: 24085192) »

See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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