Anti-Cytochrome P450 1A2 antibody (ab170204)

Overview

  • Product name
    Anti-Cytochrome P450 1A2 antibody
    See all Cytochrome P450 1A2 primary antibodies
  • Description
    Rabbit Polyclonal to Cytochrome P450 1A2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human Cytochrome P450 1A2 aa 255-282 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: P05177

  • Positive control
    • WB: Mouse liver tissue lysate. ICH-P: Human liver tissue. ICC/IF: 293 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab170204 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/10 - 1/50.
IHC-P 1/10 - 1/50.
WB 1/1000. Predicted molecular weight: 58 kDa.

Target

  • Function
    Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation. Caffeine is metabolized primarily by cytochrome CYP1A2 in the liver through an initial N3-demethylation. Also acts in the metabolism of aflatoxin B1 and acetaminophen. Participates in the bioactivation of carcinogenic aromatic and heterocyclic amines. Catalizes the N-hydroxylation of heterocyclic amines and the O-deethylation of phenacetin.
  • Tissue specificity
    Liver.
  • Sequence similarities
    Belongs to the cytochrome P450 family.
  • Cellular localization
    Endoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Aryl hydrocarbon hydroxylase antibody
    • CP 12 antibody
    • CP12 antibody
    • CP1A2_HUMAN antibody
    • CYP1A2 antibody
    • CYPIA2 antibody
    • Cytochrome P(3)450 antibody
    • Cytochrome P450 1A2 antibody
    • Cytochrome P450 4 antibody
    • Cytochrome P450 family 1 polypeptide 2 antibody
    • Cytochrome P450 family 1 subfamily A polypeptide 2 antibody
    • Cytochrome P450 subfamily I aromatic compound inducible polypeptide 2 antibody
    • Cytochrome P450-P3 antibody
    • Cytochrome P4501A2 antibody
    • Dioxin inducable P3 450 antibody
    • Flavoprotein linked monooxygenase antibody
    • Microsomal monooxygenase antibody
    • P(3)450 antibody
    • P3 450 antibody
    • P450 4 antibody
    • P450 form 4 antibody
    • P450 P3 antibody
    • P450(PA) antibody
    • Xenobiotic monooxygenase antibody
    see all

Images

  • Anti-Cytochrome P450 1A2 antibody (ab170204) + Mouse liver tissue lysate at 35 µg

    Predicted band size: 58 kDa

  • Immunocytochemistry/ Immunofluorescence analysis of 293 cells labelling Cytochrome P450 1A2 (green) with ab170204. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. Counterstained with DAPI (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling Cytochrome P450 1A2 with ab170204. A peroxidase-conjugated anti-rabbit IgG was used as the secondary antibody, followed by DAB staining.

References

ab170204 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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