Recombinant
RabMAb

Anti-Cytochrome P450 1A2 antibody [EPR6138(2)] (ab151728)

Overview

  • Product name
    Anti-Cytochrome P450 1A2 antibody [EPR6138(2)]
    See all Cytochrome P450 1A2 primary antibodies
  • Description
    Rabbit monoclonal [EPR6138(2)] to Cytochrome P450 1A2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

  • Positive control
    • Caco2, HepG2, HeLa and A549 cell lysates; HeLa cells.
  • General notes

     

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab151728 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 58 kDa.
Flow Cyt 1/1000 - 1/10000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function
      Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation. Caffeine is metabolized primarily by cytochrome CYP1A2 in the liver through an initial N3-demethylation. Also acts in the metabolism of aflatoxin B1 and acetaminophen. Participates in the bioactivation of carcinogenic aromatic and heterocyclic amines. Catalizes the N-hydroxylation of heterocyclic amines and the O-deethylation of phenacetin.
    • Tissue specificity
      Liver.
    • Sequence similarities
      Belongs to the cytochrome P450 family.
    • Cellular localization
      Endoplasmic reticulum membrane. Microsome membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Aryl hydrocarbon hydroxylase antibody
      • CP 12 antibody
      • CP12 antibody
      • CP1A2_HUMAN antibody
      • CYP1A2 antibody
      • CYPIA2 antibody
      • Cytochrome P(3)450 antibody
      • Cytochrome P450 1A2 antibody
      • Cytochrome P450 4 antibody
      • Cytochrome P450 family 1 polypeptide 2 antibody
      • Cytochrome P450 family 1 subfamily A polypeptide 2 antibody
      • Cytochrome P450 subfamily I aromatic compound inducible polypeptide 2 antibody
      • Cytochrome P450-P3 antibody
      • Cytochrome P4501A2 antibody
      • Dioxin inducable P3 450 antibody
      • Flavoprotein linked monooxygenase antibody
      • Microsomal monooxygenase antibody
      • P(3)450 antibody
      • P3 450 antibody
      • P450 4 antibody
      • P450 form 4 antibody
      • P450 P3 antibody
      • P450(PA) antibody
      • Xenobiotic monooxygenase antibody
      see all

    Images

    • Overlay histogram showing MCF7 cells stained with ab151728 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab151728, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
    • All lanes : Anti-Cytochrome P450 1A2 antibody [EPR6138(2)] (ab151728) at 1/1000 dilution

      Lane 1 : Caco2 cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : A549 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 58 kDa

    • Immunofluorescent analysis of HeLa cells labeling Cytochrome P450 1A2 with ab151728 at 1/250 dilution.

    References

    ab151728 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Unfortunately, I am sorry to confirm we did not specifically test ab151728 CYP1A antibody for this cross reactivity. However, we did a BLAST with the immunogen sequence and this indicates that theoretically this antibody will not cross react with other...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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