Anti-Cytochrome P450 26B antibody (ab79818)

Overview

  • Product name
    Anti-Cytochrome P450 26B antibody
    See all Cytochrome P450 26B primary antibodies
  • Description
    Rabbit polyclonal to Cytochrome P450 26B
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human, Xenopus laevis
  • Immunogen

    Synthetic peptide from the C terminal region of Human CYP26B1, conjugated to KLH

  • Positive control
    • 293 cell line lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab79818 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Predicted molecular weight: 58 kDa.
ELISA 1/1000.

Target

  • Function
    Involved in the metabolism of retinoic acid (RA), rendering this classical morphogen inactive through oxidation. Involved in the specific inactivation of all-trans-retinoic acid (all-trans-RA), with a preference for the following substrates: all-trans-RA > 9-cis-RA > 13-cis-RA. Generates several hydroxylated forms of RA, including 4-OH-RA, 4-oxo-RA, and 18-OH-RA. Esential for postnatal survival. Plays a central role in germ cell development: acts by degrading RA in the developing testis, preventing STRA8 expression, thereby leading to delay of meiosis. Required for the maintenance of the undifferentiated state of male germ cells during embryonic development in Sertoli cells, inducing arrest in G0 phase of the cell cycle and preventing meiotic entry. Plays a role in skeletal development, both at the level of patterning and in the ossification of bone and the establishment of some synovial joints.
  • Tissue specificity
    Highly expressed in brain, particularly in the cerebellum and pons.
  • Involvement in disease
    Radiohumeral fusions with other skeletal and craniofacial anomalies
  • Sequence similarities
    Belongs to the cytochrome P450 family.
  • Cellular localization
    Endoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CP26 antibody
    • CP26B_HUMAN antibody
    • CYP26A2 antibody
    • cyp26b1 antibody
    • Cytochrome P450 26A2 antibody
    • Cytochrome P450 26B1 antibody
    • Cytochrome P450 family 26 subfamily B polypeptide 1 antibody
    • Cytochrome P450 retinoic acid-inactivating 2 antibody
    • Cytochrome P450 retinoid metabolizing protein antibody
    • Cytochrome P450 subfamily XXVIA, polypeptide 2 antibody
    • Cytochrome P450 subfamily XXVIB polypeptide 1 antibody
    • Cytochrome P450RAI-2 antibody
    • DKFZp686G0638 antibody
    • dol antibody
    • EC 1.14. antibody
    • fc21d03 antibody
    • MGC129613 antibody
    • P450 26A2 antibody
    • P450 retinoic acid inactivating 2 antibody
    • P450RAI 2 antibody
    • P450RAI2 antibody
    • Retinoic acid metabolizing cytochrome antibody
    • Retinoic acid-metabolizing cytochrome antibody
    • RHFCA antibody
    • stocksteif antibody
    • wu:fc21d03 antibody
    • wu:fc26h10 antibody
    • zgc:76999 antibody
    see all

Images

  • Immunohistochemical staining of formalin-fixed and paraffin-embedded human brain tissue with ab79818 followed by HRP-conjugated and DAB staining.

  • Anti-Cytochrome P450 26B antibody (ab79818) at 1/300 dilution + Xenopus laevis flank ectoderm whole tissue lysate at 9 µg

    Secondary
    HRP-conjugated goat anti-rabbit IgG polyclonal at 1/5000 dilution
    Developed using the ECL technique

    Performed under non-reducing conditions.

    Predicted band size : 58 kDa
    Observed band size : 55,60 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    See Abreview

  • Anti-Cytochrome P450 26B antibody (ab79818) at 1/50 dilution + 293 cell lysate at 35 µg

    Predicted band size : 58 kDa
    Observed band size : 54 kDa (why is the actual band size different from the predicted?)

References

ab79818 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
IHC - Wholemount
Sample
Xenopus laevis Tissue (Cornea)
Specification
Cornea
Username

Abcam user community

Verified customer

Submitted Feb 05 2014

Application
Western blot
Loading amount
9 µg
Gel Running Conditions
Non-reduced Denaturing
Sample
Xenopus laevis Tissue lysate - whole (Flank ectoderm)
Specification
Flank ectoderm
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jan 24 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step
None
Sample
Xenopus laevis Tissue sections (Head ectoderm)
Specification
Head ectoderm
Permeabilization
No
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 21 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Xenopus laevis Tissue sections (Heads)
Specification
Heads
Fixative
Formaldehyde
Antigen retrieval step
None
Permeabilization
No
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 30 2012

I am very pleased to hear you would like to accept our offer and test ab79818 on Xenopus in IHC. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for on Xenopus in IHC and inclu...

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Thank you for contacting us.

Unfortunately the sequence homology between the immunogen used for ab53555 and the Xenopus protein falls below our threshold for qualifying for the abreview discount. Products must have at least an 85% homology wh...

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Thank you very much for your interest in ab79818.

There is good homology between the immunogen used to create this and the Xenopus protein (uniprot: F6S5X5_XENTR) To our knowledge, ab79818 has not been tested in Xenopus. Therefore, I can offe...

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