Anti-Cytochrome P450 2B1 + 2B2 antibody [b/e3 (9.14)] (ab22719)

Overview

  • Product nameAnti-Cytochrome P450 2B1 + 2B2 antibody [b/e3 (9.14)]
    See all Cytochrome P450 2B1 + 2B2 primary antibodies
  • Description
    Mouse monoclonal [b/e3 (9.14)] to Cytochrome P450 2B1 + 2B2
  • SpecificityThis product does not react with Rat CYP 1A1, 1A2, 2A1, 2C6, 2C7, 2C11, 4A2 and 4A3
  • Tested applicationsSuitable for: ELISA, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Rat liver cytochrome P450 2B1 and 2B2.

Applications

Our Abpromise guarantee covers the use of ab22719 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 56 kDa.
IP Use a concentration of 5 µg/ml.

Target

  • RelevanceThe Cytochrome P450 superfamily of enzymes is one of three enzyme systems which metabolize the fatty acid arachadonic acid (AA) to regulators of vascular tone. P450 enzymes are monooxygenase enzymes which require several co-factors such as nicotinamide adenine dinucleotide phosphate (NADPH) and P450 reductase. There are over 200 known genes which encode P450s. Epoxygenases are those P450s which metabolize AA to epoxyeicosatrienoic acid (EETs) and omega-hydroxylases are those P450s which produce 19- and 20-hydroxyeicosatetraenoic acids (19- and 20-HETE). As well as fatty acid metabolism, P450s also metabolize many drugs and toxins. Cytochrome P450 3A4 is abundantly expressed in liver and small intestine and is inducible by barbiturates, glucocorticoids and rifampicin.
  • Cellular localizationMembrane bound, endoplasmic reticulum
  • Database links
  • Alternative names
    • Cyp2b 1 antibody
    • Cyp2b 2 antibody
    • Cyp2b1 antibody
    • Cyp2b2 antibody
    • Cypbe antibody
    • Cype antibody
    • CYPIIB1 antibody
    • CYPIIB2 antibody
    • Cytochrome P 450b antibody
    • Cytochrome P 450b type e antibody
    • Cytochrome P 450e M antibody
    • Cytochrome P450 2B1 antibody
    • Cytochrome P450 2B2 antibody
    • Cytochrome P450 family 2 subfamily b polypeptide 1 antibody
    • Cytochrome P450 family 2 subfamily b polypeptide 2 antibody
    • Cytochrome P450 subfamily IIB phenobarbital inducible b e antibody
    • ec 1.14.14.1 antibody
    • P450 B antibody
    • P450 LM2 antibody
    • P450 PB1 and P450 PB2 antibody
    • P450 PB4 antibody
    • P450b antibody
    • P450E antibody
    see all

Anti-Cytochrome P450 2B1 + 2B2 antibody [b/e3 (9.14)] images

  • Cytochrome P450 2B1 + 2B2 was immunoprecipitated using 0.5mg Mouse Liver tissue, 5µg of Mouse monoclonal to Cytochrome P450 2B1 + 2B2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, Mouse Liver tissue lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab22719.

    Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.

    Band: 56kDa; Cytochrome P450 2B1 + 2B2

References for Anti-Cytochrome P450 2B1 + 2B2 antibody [b/e3 (9.14)] (ab22719)

ab22719 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"