Anti-Cytochrome P450 2B1 + 2B2 antibody [b/e4 (9.20)] (ab22721)


  • Product nameAnti-Cytochrome P450 2B1 + 2B2 antibody [b/e4 (9.20)]
    See all Cytochrome P450 2B1 + 2B2 primary antibodies
  • Description
    Mouse monoclonal [b/e4 (9.20)] to Cytochrome P450 2B1 + 2B2
  • SpecificityClone b/e4 does not react with CYP 1A1, 1A2, 2C6, 2C7, 2C11, 4A2, 4A3.
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Full length protein (Rat).


Our Abpromise guarantee covers the use of ab22721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 56 kDa.


  • RelevanceThe Cytochrome P450 superfamily of enzymes is one of three enzyme systems which metabolize the fatty acid arachadonic acid (AA) to regulators of vascular tone. P450 enzymes are monooxygenase enzymes which require several co-factors such as nicotinamide adenine dinucleotide phosphate (NADPH) and P450 reductase. There are over 200 known genes which encode P450s. Epoxygenases are those P450s which metabolize AA to epoxyeicosatrienoic acid (EETs) and omega-hydroxylases are those P450s which produce 19- and 20-hydroxyeicosatetraenoic acids (19- and 20-HETE). As well as fatty acid metabolism, P450s also metabolize many drugs and toxins. Cytochrome P450 3A4 is abundantly expressed in liver and small intestine and is inducible by barbiturates, glucocorticoids and rifampicin.
  • Cellular localizationMembrane bound, endoplasmic reticulum
  • Database links
  • Alternative names
    • Cyp2b 1 antibody
    • Cyp2b 2 antibody
    • Cyp2b1 antibody
    • Cyp2b2 antibody
    • Cypbe antibody
    • Cype antibody
    • CYPIIB1 antibody
    • CYPIIB2 antibody
    • Cytochrome P 450b antibody
    • Cytochrome P 450b type e antibody
    • Cytochrome P 450e M antibody
    • Cytochrome P450 2B1 antibody
    • Cytochrome P450 2B2 antibody
    • Cytochrome P450 family 2 subfamily b polypeptide 1 antibody
    • Cytochrome P450 family 2 subfamily b polypeptide 2 antibody
    • Cytochrome P450 subfamily IIB phenobarbital inducible b e antibody
    • ec antibody
    • P450 B antibody
    • P450 LM2 antibody
    • P450 PB1 and P450 PB2 antibody
    • P450 PB4 antibody
    • P450b antibody
    • P450E antibody
    see all

Anti-Cytochrome P450 2B1 + 2B2 antibody [b/e4 (9.20)] images

  • All lanes : Anti-Cytochrome P450 2B1 + 2B2 antibody [b/e4 (9.20)] (ab22721) at 1/1500 dilution

    Lane 1 : Male Rat liver tissue lysate
    Lane 2 : Male Rat liver tissue lysate - phenobarbital induced (100mg/kg for 24 hours)

    Lysates/proteins at 20 µg per lane.

    HRP-conjugated anti-mouse IgG polyclonal at 1/3000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 56 kDa
    Observed band size : 62 kDa (why is the actual band size different from the predicted?)

    Exposure time : 5 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-Cytochrome P450 2B1 + 2B2 antibody [b/e4 (9.20)] (ab22721)

This product has been referenced in:
  • Probert PM  et al. Utility of B-13 progenitor-derived hepatocytes in hepatotoxicity and genotoxicity studies. Toxicol Sci 137:350-70 (2014). WB ; Rat . Read more (PubMed: 24235770) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Rat Tissue lysate - whole (Male rat liver)
Specification Male rat liver
Treatment 100mg/kg phenobarbital for 24hrs
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C

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Verified customer

Submitted Aug 14 2013

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Both mentioned antibodies have been validated to react with rat species. At Abcam, our aim is to share all the information we have available from our products in the Website, to help customers decide the best opti...

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