Overview

  • Product nameAnti-Cytochrome P450 2B6 antibody
    See all Cytochrome P450 2B6 primary antibodies
  • Description
    Rabbit polyclonal to Cytochrome P450 2B6
  • Tested applicationsSuitable for: ICC/IF, ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from an internal region of human Cytochrome P450 2B6.

  • Positive control
    • Extracts from HT-29 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab69652 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
ELISA 1/10000.
IHC-P Use a concentration of 5 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).

Target

  • FunctionCytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
  • Tissue specificityExpressed in liver, lung and heart right ventricle.
  • Sequence similaritiesBelongs to the cytochrome P450 family.
  • Post-translational
    modifications
    Phosphorylation is accompanied by a decrease in enzyme activity.
  • Cellular localizationEndoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1,4 cineole 2 exo monooxygenase antibody
    • 11B1 antibody
    • CP2B6_HUMAN antibody
    • CPB 6 antibody
    • CPB6 antibody
    • CYP 2B6 antibody
    • CYP IIB6 antibody
    • CYP2B antibody
    • CYP2B6 antibody
    • CYP2B7 antibody
    • CYP2B7P antibody
    • CYPIIB6 antibody
    • Cytochrome P450 2B6 antibody
    • Cytochrome P450 family 2 subfamily B antibody
    • Cytochrome P450 family 2 subfamily B polypeptide 6 antibody
    • Cytochrome P450 IIB1 antibody
    • Cytochrome P450 phenobarbital inducible antibody
    • Cytochrome P450 subfamily IIB (phenobarbital inducible) antibody
    • Cytochrome P450 subfamily IIB (phenobarbital inducible) polypeptide 6 antibody
    • Cytochrome P450 subfamily IIB polypeptide 6 antibody
    • EFVM antibody
    • IIB1 antibody
    • P450 antibody
    • P450 IIB1 antibody
    • P450IIB1 antibody
    see all

Anti-Cytochrome P450 2B6 antibody images

  • All lanes : Anti-Cytochrome P450 2B6 antibody (ab69652) at 1/500 dilution

    Lane 1 : Extracts from HT-29 cells
    Lane 2 : Extracts from HT-29 cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 56 kDa
    Observed band size : 56 kDa
    Additional bands at : 67 kDa,74 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab69652 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab69652, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab69652 staining in Human Breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab69652, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-Cytochrome P450 2B6 antibody (ab69652)

This product has been referenced in:
  • Shah A  et al. HIV gp120- and methamphetamine-mediated oxidative stress induces astrocyte apoptosis via cytochrome P450 2E1. Cell Death Dis 4:e850 (2013). Human . Read more (PubMed: 24113184) »

See 1 Publication for this product

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Thank you for your enquiry which has been forwarded to the scientific support team. We can be contacted at technical@abcam.com. I am pleased to provide the WB testing protocol for ab69652 Cytochrome P450 2B6 antibody. Please not, this is a guideline...

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