Overview

  • Product nameAnti-Cytochrome P450 2C11 antibody
  • Description
    Rabbit polyclonal to Cytochrome P450 2C11
  • Tested applicationsSuitable for: IHC-P, ICC/IF, ELISA, ICC, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Rat, Cat, Human
  • Immunogen

    This product was produced with the following immunogens:
    Synthetic peptide corresponding to Rat Cytochrome P450 2C11 aa 49-60.
    Sequence: DIGQSIKKFSKV

    Synthetic peptide corresponding to Rat Cytochrome P450 2C11 aa 491-500.
    Sequence:

    QRADSLSSHL

Properties

Applications

Our Abpromise guarantee covers the use of ab3571 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/20 - 1/200.
ELISA Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
IHC-Fr 1/100.

Immunohistochemical staining of P450 2C11 in rat brain results in perivascular staining.

WB 1/1500.

By Western blot, this antibody detects an ~50 kDa protein representing P450 2C11 from rat liver extract.

Target

  • FunctionMetabolizes testosterone mainly in positions 2 alpha and 16 alpha.
  • Tissue specificityLiver; male-specific.
  • Sequence similaritiesBelongs to the cytochrome P450 family.
  • Cellular localizationEndoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CP2CB_RAT antibody
    • Cyp2c antibody
    • Cyp2c11 antibody
    • CYP2CII antibody
    • CYPIIC11 antibody
    • Cytochrome P-450(M-1) antibody
    • Cytochrome P450 2C11 antibody
    • Cytochrome P450-UT-2 antibody
    • Cytochrome P450-UT-A antibody
    • Cytochrome P450H antibody
    • P450 UT A antibody
    • P450H antibody
    • UT2 antibody
    see all

Anti-Cytochrome P450 2C11 antibody images

  • ab3571 staining Cytochrome P450 2C11 (green) in HeLa cells (right), compared to control (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-rabbit was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.

  • ab3571 staining Cytochrome P450 2C11 (green) in PC12 cells (right), compared to control (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-rabbit was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.

  • ab3571 staining Cytochrome P450 2C11 (green) in H-4-II-E cells (right), compared to control (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-rabbit was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.

  • ab3571 staining Cytochrome P450 2C11 in the cytoplasm of Rat liver tissue (right) compared with a negative control in the absence of primary antibody (left) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Tissues were then blocked in 3% H2O2-methanol for 15 min at room temperature. Sections were incubated with primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • ab3571 staining Cytochrome P450 2C11 in the cytoplasm of Rat kidney tissue (right) compared with a negative control in the absence of primary antibody (left) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Tissues were then blocked in 3% H2O2-methanol for 15 min at room temperature. Sections were incubated with primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • IHC image of ab3571 staining in human renal carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3571, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-Cytochrome P450 2C11 antibody (ab3571)

This product has been referenced in:
  • El-Sherbeni AA & El-Kadi AO Alterations in cytochrome P450-derived arachidonic acid metabolism during pressure overload-induced cardiac hypertrophy. Biochem Pharmacol 87:456-66 (2014). Rat . Read more (PubMed: 24300133) »
  • Clarke JD  et al. Experimental nonalcoholic steatohepatitis increases exposure to simvastatin hydroxy acid by decreasing hepatic organic anion transporting polypeptide expression. J Pharmacol Exp Ther 348:452-8 (2014). Read more (PubMed: 24403518) »

See all 4 Publications for this product

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