The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
RelevanceThe cytochrome P450 superfamily of heme-thiolate mono-oxygenases play a key role in the oxidative metabolism of many endogenous and exogenous molecules and are classified by their primary structure into four main groups. It is an ancient superfamily, which has been identified in prokaryotes and in many types of eukaryotic cells. Cytochrome P450 2 family, (CYP2) is the largest of the four groups, having five sub-families, 2A-2E. CYP2C23 was reported to efficiently catalyze arachidonic acid to epoxyeicosatrienoic acids (EETs) in rat kidney.
Western blot - Cytochrome P450 2C23 antibody (ab53944)
All lanes : Anti-Cytochrome P450 2C23 antibody (ab53944) at 1/2000 dilution
Lane 1 : Control liver microsome Lane 2 : Phenobarbital-treated liver microsome
Lysates/proteins at 10 µg per lane.
Predicted band size : 56 kDa Observed band size : 56 kDa
References for Anti-Cytochrome P450 2C23 antibody (ab53944)
This product has been referenced in:
Caligiuri SP et al. Dietary linoleic acid and a-linolenic acid differentially affect renal oxylipins and phospholipid fatty acids in diet-induced obese rats. J Nutr143:1421-31 (2013).
Read more (PubMed: 23902961) »
Mustafa S et al. Renal expression of arachidonic acid metabolizing enzymes and RhoA/Rho kinases in fructose insulin resistant hypertensive rats. Mol Cell Biochem333:203-9 (2010).
Read more (PubMed: 19633817) »