Overview

  • Product name
    Anti-Cytochrome P450 2C6 antibody [K1]
  • Description
    Mouse monoclonal [K1] to Cytochrome P450 2C6
  • Specificity
    Cross reacts with human CYp 2C9 and as yet unidentified Human and Mouse CYP 2Cs. Clone K1 does not react with human, rat and mouse CYP 2C8
  • Tested applications
    Suitable for: ICC/IF, ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Full length protein from liver (Rat).

Properties

Applications

Our Abpromise guarantee covers the use of ab22723 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 5 µg/ml.
ELISA Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 56 kDa.

Target

  • Relevance
    Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
  • Cellular localization
    Membrane-bound; Endoplasmic reticulum.
  • Database links
  • Alternative names
    • Cyp2c6 antibody
    • CYPIIC6 antibody
    • P450 PB1 antibody
    • PB1 antibody
    • PTF 2 antibody
    see all

Images

  • ICC/IF image of ab22723 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22723, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Abdellatef SA  et al. The effect of physical and chemical cues on hepatocellular function and morphology. Int J Mol Sci 15:4299-317 (2014). ICC/IF ; Human . Read more (PubMed: 24619224) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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