Anti-Cytochrome P450 2C8 + 2C9 + 2C19 + 2C12 antibody (ab22596)

Overview

  • Product name
    Anti-Cytochrome P450 2C8 + 2C9 + 2C19 + 2C12 antibody
  • Description
    Rabbit polyclonal to Cytochrome P450 2C8 + 2C9 + 2C19 + 2C12
  • Tested applications
    Suitable for: IHC-M, WB, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide (Human) conjugated to KLH by a MBS linker.

  • Positive control
    • Rat liver microsomal preparations and rat brain

Properties

Applications

Our Abpromise guarantee covers the use of ab22596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-M
WB
IHC-Fr
IHC-P
  • Application notes
    IHC-P: 1/1000. Antigen retrieval is not essential but may optimise staining.
    IHC-Fr: 1/1000.
    IHC-M: 1/1000.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 56 kDa.
    Dilute to working strength with 50mM Tris-HCl buffer (pH 7.6) containing 1.5% sodium chloride and 1% normal goat serum (if a goat anti-rabbit IgG linker antibody is to be used). Store diluted antibody at 2-4°C and use within 1 month.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      The cytochrome P450 superfamily of heme-thiolate mono-oxygenases play a key role in the oxidative metabolism of many endogenous and exogenous molecules and are classified by their primary structure into four main groups. It is an ancient superfamily, which has been identified in prokaryotes and in many types of eukaryotic cells. Cytochrome P450 2 family, (CYP2) is the largest of the four groups, having five sub-families, 2A-2E. Anti-depressants are metabolised by, and are competitive inhibitors of, several CYP2C isozymes including CYP2C8, 2C9 and 2C19 amongst others. The non-steroidal anti-inflammatory drug indomethacin has recently been shown to be o-demethylated exclusively by CYP2C9 in humans. Rat CYP2C12, which shares a common C-terminal amino acid sequence with 2C8, 2C9 and 2C19, is a sex-dependent steroid hydroxylase present in liver which is transcriptionally activated by the continuous plasma growth hormone profile characteristic of adult female rats.
    • Cellular localization
      Membrane-bound. Endoplasmic reticulum
    • Database links
    • Alternative names
      • CPC12 antibody
      • CPC8 antibody
      • CPC9 antibody
      • CPCJ antibody
      • CYP2C antibody
      • CYP2C10 antibody
      • P450 PB1 antibody
      • P450C2C antibody
      • P450IIC19 antibody
      • P450IIC9 antibody
      see all

    Anti-Cytochrome P450 2C8 + 2C9 + 2C19 + 2C12 antibody images

    • Immunohistochemical analysis of frozen Human heart tissue, staining Cytochrome P450 2C8 + 2C9 + 2C19 + 2C12 with ab22596. A biotinylated anti-rabbit IgG was used as the secondary antibody. Staining was detected using DAB.

    References for Anti-Cytochrome P450 2C8 + 2C9 + 2C19 + 2C12 antibody (ab22596)

    This product has been referenced in:
    • Shao Z  et al. Cytochrome P450 2C8 ?3-long-chain polyunsaturated fatty acid metabolites increase mouse retinal pathologic neovascularization--brief report. Arterioscler Thromb Vasc Biol 34:581-6 (2014). IHC - Wholemount, WB ; Mouse . Read more (PubMed: 24458713) »
    • Michaud V  et al. Metabolic activity and mRNA levels of human cardiac CYP450s involved in drug metabolism. PLoS One 5:e15666 (2010). IHC-Fr ; Human . Read more (PubMed: 21179487) »

    See all 3 Publications for this product

    Product Wall

    Thank you for your enquiry. I am sorry to hear that you have been having problems with this antibody. Following correspondence with the source of this antibody unfortunately I have been unable to obtain an image of this antibody applied using IHC-P...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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