Anti-Cytochrome P450 2C8 + 2C9 + 2C19 + 2C12 antibody (ab22596)


  • Product nameAnti-Cytochrome P450 2C8 + 2C9 + 2C19 + 2C12 antibody
    See all Cytochrome P450 2C8 + 2C9 + 2C19 + 2C12 primary antibodies
  • Description
    Rabbit polyclonal to Cytochrome P450 2C8 + 2C9 + 2C19 + 2C12
  • Tested applicationsSuitable for: IHC-M, WB, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide (Human) conjugated to KLH by a MBS linker.

  • Positive control
    • Rat liver microsomal preparations and rat brain



Our Abpromise guarantee covers the use of ab22596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesIHC-P: 1/1000. Antigen retrieval is not essential but may optimise staining.
    IHC-Fr: 1/1000.
    IHC-M: 1/1000.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 56 kDa.
    Dilute to working strength with 50mM Tris-HCl buffer (pH 7.6) containing 1.5% sodium chloride and 1% normal goat serum (if a goat anti-rabbit IgG linker antibody is to be used). Store diluted antibody at 2-4°C and use within 1 month.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceThe cytochrome P450 superfamily of heme-thiolate mono-oxygenases play a key role in the oxidative metabolism of many endogenous and exogenous molecules and are classified by their primary structure into four main groups. It is an ancient superfamily, which has been identified in prokaryotes and in many types of eukaryotic cells. Cytochrome P450 2 family, (CYP2) is the largest of the four groups, having five sub-families, 2A-2E. Anti-depressants are metabolised by, and are competitive inhibitors of, several CYP2C isozymes including CYP2C8, 2C9 and 2C19 amongst others. The non-steroidal anti-inflammatory drug indomethacin has recently been shown to be o-demethylated exclusively by CYP2C9 in humans. Rat CYP2C12, which shares a common C-terminal amino acid sequence with 2C8, 2C9 and 2C19, is a sex-dependent steroid hydroxylase present in liver which is transcriptionally activated by the continuous plasma growth hormone profile characteristic of adult female rats.
    • Cellular localizationMembrane-bound. Endoplasmic reticulum
    • Database links
    • Alternative names
      • CPC12 antibody
      • CPC8 antibody
      • CPC9 antibody
      • CPCJ antibody
      • CYP2C antibody
      • CYP2C10 antibody
      • P450 PB1 antibody
      • P450C2C antibody
      • P450IIC19 antibody
      • P450IIC9 antibody
      see all

    Anti-Cytochrome P450 2C8 + 2C9 + 2C19 + 2C12 antibody images

    • Immunohistochemical analysis of frozen Human heart tissue, staining Cytochrome P450 2C8 + 2C9 + 2C19 + 2C12 with ab22596. A biotinylated anti-rabbit IgG was used as the secondary antibody. Staining was detected using DAB.

    References for Anti-Cytochrome P450 2C8 + 2C9 + 2C19 + 2C12 antibody (ab22596)

    This product has been referenced in:
    • Michaud V  et al. Metabolic activity and mRNA levels of human cardiac CYP450s involved in drug metabolism. PLoS One 5:e15666 (2010). IHC-Fr ; Human . Read more (PubMed: 21179487) »
    • Meissonnier GM  et al. Subclinical doses of T-2 toxin impair acquired immune response and liver cytochrome P450 in pigs. Toxicology 247:46-54 (2008). Read more (PubMed: 18355953) »

    See all 2 Publications for this product

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    Thank you for your enquiry. I am sorry to hear that you have been having problems with this antibody. Following correspondence with the source of this antibody unfortunately I have been unable to obtain an image of this antibody applied using IHC-P...

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