The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesWB: Use at an assay dependent dilution. Predicted molecular weight: 57 kDa.
Note: to dilute ab22590, use the following solution: 50mM Tris HCl buffer (pH 7.6) containing 1.5% sodium chloride and 1% normal goat serum (if a goat anti rabbit IgG linker antibody is to be used).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceThe cytochrome P450 superfamily of heme thiolate mono oxygenases play a key role in the oxidative metabolism of many endogenous and exogenous molecules, and are classified by their primary structure into four main groups. It is an ancient superfamily, which has been identified in prokaryotes and in many types of eukaryotic cells.
The CYP2A sub family is found in the liver, and recently in the nasal mucosa where they are involved in the metabolism of inhaled xenobiotics and nicotine. CYP2B proteins are found in the liver, where they are involved in the metabolism of hepatic drugs, and in the lung and brain, whereas the CYP2C proteins are involved in the metabolism of anti depressants. CYP2E1 is alcohol inducible, and found principally in the liver.
Rat CYP2D1, the homologue of human CYP2D6, has been shown to catalyse the conversion of hydrocodone to hydromorphone, and in rat liver microsomes it participates in the conversion of ethylmorphine to codeine and morphine. Its regional distribution in the rat brain has been described. Mutation in the cyp2D6 gene has been reported to be associated with PD10.