Overview

  • Product nameAnti-Cytochrome P450 2D6 antibody
    See all Cytochrome P450 2D6 primary antibodies
  • Description
    Rabbit polyclonal to Cytochrome P450 2D6
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse, Guinea pig, Cow, Cat, Pig, Chimpanzee, Zebrafish
  • Immunogen

    A region within synthetic peptide: ILGFGPRSQG VFLARYGPAW REQRRFSVST LRNLGLGKKS LEQWVTEEAA, corresponding to N terminal amino acids 109-158 of Human Cytochrome P450 2D6

  • Positive control
    • Human Fetal liver cell lysate, human kidney tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab62204 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 56, 37 kDa (predicted molecular weight: 56 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • RelevanceCytochrome P450 2D6 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is known to metabolize as many as 20% of commonly prescribed drugs. Its substrates include debrisoquine, an adrenergic-blocking drug; sparteine and propafenone, both anti-arrythmic drugs; and amitryptiline, an anti-depressant. The gene is highly polymorphic in the population; certain alleles result in the poor metabolizer phenotype, characterized by a decreased ability to metabolize the enzyme's substrates. Cytochrome P450 2D6 is the molecular target of autoantibodies against the "liver kidney microsomal antigen 1" (LKM 1).
  • Cellular localizationEndoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
  • Database links
  • Alternative names
    • CPD6 antibody
    • CYP2D antibody
    • CYP2D6 antibody
    • CYP2D7AP antibody
    • CYP2D7BP antibody
    • CYP2D7P2 antibody
    • CYP2D8P2 antibody
    • CYP2DL1 antibody
    • CYPIID6 antibody
    • Cytochrome P450 DB1 antibody
    • Cytochrome P450 family 2 subfamily D polypeptide 6 antibody
    • Debrisoquine 4 hydroxylase antibody
    • Flavoprotein linked monooxygenase antibody
    • Microsomal monooxygenase antibody
    • P450 DB1 antibody
    • P450C2D antibody
    • P450DB1 antibody
    • Xenobiotic monooxygenase antibody
    see all

Anti-Cytochrome P450 2D6 antibody images

  • Anti-Cytochrome P450 2D6 antibody (ab62204) at 2.5 µg/ml + Fetal liver cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG 1/ 50,000 - 1/100,000 dilution

    Predicted band size : 56 kDa
    Observed band size : 56 kDa
    Additional bands at : 37 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Cytochrome P450 2D6 with ab62204 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

  • ICC/IF image of ab62204 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62204, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Cytochrome P450 2D6 antibody (ab62204)

This product has been referenced in:
  • Shah A  et al. HIV gp120- and methamphetamine-mediated oxidative stress induces astrocyte apoptosis via cytochrome P450 2E1. Cell Death Dis 4:e850 (2013). Human . Read more (PubMed: 24113184) »

See 1 Publication for this product

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