The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.02 - 0.5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 57 kDa).
Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. Inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms.
ab106799 (3.8µg/ml) staining of paraffin embedded Human Liver shows ER-like staining in hepatocytes. Tissue treated as follows: Steamed antigen retrieval with citrate buffer pH 6 and decection using AP-staining.