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Full length native protein (purified) (Rat)
The antibody has been shown to inhibit microsomal benzene metabolism
Our Abpromise guarantee covers the use of ab28146 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||1/300. PubMed: 17021035|
|WB||1/5000. Predicted molecular weight: 50-55 kDa. This dilution was sufficient for detection of Cytochrome P450 in 20ug of human liver microsomes by colorimetric immunoblot analysis using Goat anti-Rabbit IgG:AP as the secondary antibody.|
|IHC-P||Use at an assay dependent concentration. PubMed: 24155903|
ab28146 staining cells of rat liver sections by IHC-Fr. Cells were fixed in acetone and blocked with 1% BSA for 5 minutes at 25°C prior to incubating with ab28146 diluted 1/300 for 30 minutes at 25°C. An Alexa Fluor® 594 conjugated goat anti-rabbit antibody was used as the secondary.
Double IF labeling with CYP2E1 (Red) and dipeptidyl peptidase IV (Green)
Image courtesy of an anonymous Abreview.