Anti-Cytochrome P450 2E1 antibody (ab53945)
Key features and details
- Rabbit polyclonal to Cytochrome P450 2E1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Cytochrome P450 2E1 antibody
See all Cytochrome P450 2E1 primary antibodies -
Description
Rabbit polyclonal to Cytochrome P450 2E1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide (Human) Cytochrome P450 2E1
-
Positive control
- IHC-P: Human liver carcinoma WB: Pyridine-treated kidney microsome.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Constituent: PBS -
Concentration information loading...
-
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab53945 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
|
|
IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
Notes |
---|
WB
1/1000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa). |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. Inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms. -
Sequence similarities
Belongs to the cytochrome P450 family. -
Cellular localization
Endoplasmic reticulum membrane. Microsome membrane. - Information by UniProt
-
Database links
- Entrez Gene: 1571 Human
- Omim: 124040 Human
- SwissProt: P05181 Human
- Unigene: 12907 Human
-
Alternative names
- 4-nitrophenol 2-hydroxylase antibody
- CP2E1_HUMAN antibody
- CPE1 antibody
see all
Images
-
All lanes : Anti-Cytochrome P450 2E1 antibody (ab53945) at 1/1000 dilution
Lane 1 : Control kidney microsome
Lane 2 : Pyridine-treated kidney microsome
Lysates/proteins at 10 µg per lane.
Predicted band size: 57 kDa
Observed band size: 57 kDa -
IHC image of ab53945 staining in human liver carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab53945, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
-
Datasheet download
References (4)
ab53945 has been referenced in 4 publications.
- Kaut O et al. Epigenome-Wide Analysis of DNA Methylation in Parkinson's Disease Cortex. Life (Basel) 12:N/A (2022). PubMed: 35454993
- Santra S et al. Expression of type I collagen in response to Isoniazid exposure is indirect and is facilitated by collateral induction of cytochrome P450 2E1: An in-vitro study. PLoS One 15:e0236992 (2020). PubMed: 32735603
- Feng R et al. Acetaminophen-induced liver injury is attenuated in transgenic fat-1 mice endogenously synthesizing long-chain n-3 fatty acids. Biochem Pharmacol 154:75-88 (2018). PubMed: 29679557
- Liu T et al. The CYP2E1 inhibitor DDC up-regulates MMP-1 expression in hepatic stellate cells via an ERK1/2- and Akt-dependent mechanism. Biosci Rep 33:N/A (2013). PubMed: 23577625