Anti-Cytochrome P450 2E1 antibody (ab90564)
- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-Cytochrome P450 2E1 antibody
See all Cytochrome P450 2E1 primary antibodies -
DescriptionMouse polyclonal to Cytochrome P450 2E1
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Host speciesMouse
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Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
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Species reactivityReacts with: Human
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Immunogen
Full length Human Cytochrome P450 2E1 (NP_000764.1).
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Positive control
- Human liver lysate, Cytochrome P450 2E1 transfected 293T cell lysate, Human liver tissue and HeLa cells
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: None
Constituents: 1X PBS, pH 7.2 -
Concentration information loading... -
PurityProtein A purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab90564 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | ||
| IHC-P | ||
| ICC/IF |
Application notes
ICC/IF: Use at a concentration of 10 µg/ml.
IHC-P: Use at a concentration of 3 µg/ml.
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 57 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at a concentration of 3 µg/ml.
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 57 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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FunctionMetabolizes several precarcinogens, drugs, and solvents to reactive metabolites. Inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms.
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Sequence similaritiesBelongs to the cytochrome P450 family.
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Cellular localizationEndoplasmic reticulum membrane. Microsome membrane.
- Information by UniProt
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Database links
- Entrez Gene: 1571 Human
- Omim: 124040 Human
- SwissProt: P05181 Human
- Unigene: 12907 Human
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Alternative names
- 4-nitrophenol 2-hydroxylase antibody
- CP2E1_HUMAN antibody
- CPE1 antibody
see all
Images
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Western blot - Anti-Cytochrome P450 2E1 antibody (ab90564)Anti-Cytochrome P450 2E1 antibody (ab90564) at 1 µg/ml + Human liver lysate at 50 µg
Predicted band size: 57 kDa -
Western blot - Anti-Cytochrome P450 2E1 antibody (ab90564)All lanes : Anti-Cytochrome P450 2E1 antibody (ab90564) at 1 µg/ml
Lane 1 : Cytochrome P450 2E1 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 57 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome P450 2E1 antibody (ab90564)ab90564 at 10 µg/ml staining Cytochrome P450 2E1 in permeabilised HeLa cells by Immunofluorescence.
Protocols
References
This product has been referenced in:
- Zhang X et al. A tryptophan derivative, ITE, enhances liver cell metabolic functions in vitro. Int J Mol Med 39:101-112 (2017). Read more (PubMed: 27959388) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"