Cytochrome P450 2E1 overexpression 293T lysate (whole cell) (ab94254)

Overview

  • Product nameCytochrome P450 2E1 overexpression 293T lysate (whole cell)
  • General notesab94254 is a 293T cell transfected lysate in which Human Cytochrome P450 2E1 has been transiently over-expressed using a pCMV-Cytochrome P450 2E1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
  • Tested applicationsSuitable for: WBmore details

Properties

  • BackgroundFunction: Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. Inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms. Similarity: Belongs to the cytochrome P450 family.

Associated products

Applications

Our Abpromise guarantee covers the use of ab94254 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

Cytochrome P450 2E1 overexpression 293T lysate (whole cell) images

  • ab94254 at 15µg/lane on an SDS-PAGE gel
  • All lanes : Anti-Cytochrome P450 2E1 antibody (ab90564) at 1/500 dilution

    Lane 1 : Cytochrome P450 2E1 overexpression 293T lysate (whole cell) (ab94254)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References for Cytochrome P450 2E1 overexpression 293T lysate (whole cell) (ab94254)

ab94254 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"