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Rabbit polyclonal to Cytochrome p450 2J2
Recombinant fragment, corresponding to a region within amino acids 213-472 of Human Cytochrome p450 2J2 (Uniprot ID: P51589).
Human breast cancer tissue; H1299, A431, HeLa, HepG2 whole cell lysate
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 58 kDa.
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
This enzyme metabolizes arachidonic acid predominantly via a NADPH-dependent olefin epoxidation to all four regioisomeric cis-epoxyeicosatrienoic acids. One of the predominant enzymes responsible for the epoxidation of endogenous cardiac arachidonic acid pools.
Highly expressed in heart, present at lower levels in liver, ileum, jejunum, colon, and kidney.
Belongs to the cytochrome P450 family.
Endoplasmic reticulum membrane. Microsome membrane.
Information by UniProt
Arachidonic acid epoxygenase antibody
Western blot - Anti-Cytochrome p450 2J2 antibody (ab151996)
All lanes : Anti-Cytochrome p450 2J2 antibody (ab151996) at 1/1000 dilution Lane 1 : A431 whole cell lysate Lane 2 : H1299 whole cell lysate Lysates/proteins at 30 µg per lane. Predicted band size: 58 kDa 7.5% SDS PAGE
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome p450 2J2 antibody (ab151996)
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling Cytochrome p450 2J2 with ab151996 at 1/500 dilution.
has not yet been referenced specifically in any publications.
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