Anti-Cytochrome p450 2J2 antibody (ab151996)

Overview

  • Product name
    Anti-Cytochrome p450 2J2 antibody
    See all Cytochrome p450 2J2 primary antibodies
  • Description
    Rabbit polyclonal to Cytochrome p450 2J2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 213-472 of Human Cytochrome p450 2J2 (Uniprot ID: P51589).

  • Positive control
    • Human breast cancer tissue; H1299, A431, HeLa, HepG2 whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab151996 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 58 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    This enzyme metabolizes arachidonic acid predominantly via a NADPH-dependent olefin epoxidation to all four regioisomeric cis-epoxyeicosatrienoic acids. One of the predominant enzymes responsible for the epoxidation of endogenous cardiac arachidonic acid pools.
  • Tissue specificity
    Highly expressed in heart, present at lower levels in liver, ileum, jejunum, colon, and kidney.
  • Sequence similarities
    Belongs to the cytochrome P450 family.
  • Cellular localization
    Endoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Arachidonic acid epoxygenase antibody
    • CP2J2_HUMAN antibody
    • CPJ2 antibody
    • CYP2J2 antibody
    • CYPIIJ2 antibody
    • Cytochrome P450 2J2 antibody
    • Cytochrome P450 family 2 subfamily J polypeptide 2 antibody
    • Cytochrome P450 subfamily IIJ (arachidonic acid epoxygenase) polypeptide 2 antibody
    • Cytochrome P450 subfamily IIJ polypeptide 2 antibody
    • Flavoprotein linked monooxygenase antibody
    • Microsomal monooxygenase antibody
    • OTTHUMP00000010550 antibody
    see all

Images

  • All lanes : Anti-Cytochrome p450 2J2 antibody (ab151996) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : H1299 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 58 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling Cytochrome p450 2J2 with ab151996 at 1/500 dilution.

References

ab151996 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK293T)
Permeabilization
Yes - Triton-X
Specification
HEK293T
Blocking step
BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Fixative
Paraformaldehyde
Username

Kavin Abelak

Verified customer

Submitted Apr 12 2016

Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (breast lines cells)
Total protein in input
2000 µg
Immuno-precipitation step
Protein A
Specification
breast lines cells
Username

Abcam user community

Verified customer

Submitted Oct 06 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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